目的 建立疲王颗粒中人参皂苷Rg₁、Re、Rb₁的定量测定方法。方法 采用高效液相色谱-蒸发光散射检测(HPLC-ELSD)法，色谱柱为Shim-pack VP-ODS(150 mm×4.6 mm，5 μm)，流动相为乙腈-水，梯度洗脱：0～20 min，20%乙腈；20～35 min，20%～30%乙腈；35～55 min，30%乙腈；55～56 min，30%～45%乙腈；体积流量0.8 mL/min；柱温25℃；ELSD漂移管温度100℃，气体流量3 L/min。结果 人参皂苷Rg₁、Re、Rb₁的线性范围分别为125.44～470.40 μg/mL(r=0.999 6)、88.96～333.60 μg/mL(r=0.999 2)、157.12～589.20 μg/mL(r=0.999 5)，其平均加样回收率分别为100.27%(RSD为1.713%，n=9)、101.29%(RSD为1.220%，n=9)、101.00%(RSD为1.668%，n=9)。结论 该方法测定简便、快速、重复性好，可作为疲王颗粒的质量控制方法。

Objective To establish a method for the content determination of ginsenoside Rg₁, Re, and Rb₁ in Piwang Granules. Methods HPLC-ELSD was used. The analysis was carried on Shim-pack VP-ODS (150 mm × 4.6 mm, 5 μm) column with mobile phase consisted of acetoneitrile-water, and the gradient elution ( 0—20 min, acetoneitrile 20%, 20－35 min, acetoneitrile 20%→30%, 35－55 min, acetoneitrile 30%, 55－56 min, acetoneitrile 30%→45%) was used. The flow rate was 0.8 mL/min. The column temperature was 25℃. The drift tube temperature was 100℃. The flow rate of gas was 3 L/min. Results The responses of ginsenoside Rg₁, Re, and Rb₁ were liner in the ranges of 125.44－470.40 (r=0.999 6), 88.96－333.60 (r=0.999 2), and 157.12－589.20 μg/mL (r=0.999 5), respectively. The average recoveries were 100.27% (RSD =1.713%, n=9), 101.29% (RSD=1.220%, n=9), and 101.00% (RSD =1.668%, n=9). Conclusion The method is simple, rapid, and with good repeatability. It can be used for the quality control of Piwang Granules.

国家“十五”科技攻关项目（奥运专项 中药消除运动性疲劳的应用研究2002BA904B03）