目的 研究东北岩高兰Empetrum nigrum var. japonicum全草的化学成分。方法 采用硅胶、MCI、Sephadex LH-20、半制备HPLC等柱色谱方法进行了分离纯化，并根据化合物的理化性质和波谱分析等方法鉴定其结构。结果 从东北岩高兰95%乙醇提取物中分离得到12个化合物，分别鉴定为3β-羟基-21-烯-岩高兰三萜（1）、4’-羟基-α，β-二氢查耳酮-2’-O-β-D-葡萄糖苷（2）、5-（2-苯乙基）-间苯二酚-1-O-β-D-葡萄糖苷（3）、2’，4’-二羟基查耳酮（4）、2’-甲氧基-4’-羟基-α，β-二氢查耳酮（5）、苯丙酸（6）、肉桂醇（7）、槲皮素（8）、（-）-表儿茶素（9）、槲皮素-3-O-α-L-阿拉伯糖苷（10）、金丝桃苷（11）、8-甲氧基槲皮素-3-葡萄糖苷（12）。结论 化合物1为1个新的三萜，化合物2为1个新的二氢查耳酮，化合物3、4、6～12为首次从岩高兰属植物中分离得到。

Objective To study the chemical constituents from the herbs of Empetrum nigrum var. japonicum. Methods The compounds were isolated and purified by silica gel, MCI gel, Sephadex LH-20 column chromatographies, semi-preparative HPLC, and their structures were mainly elucidated on the basis of physicochemical characteristics and spectral analysis. Results Twelve compounds were isolated from 95% EtOH extract of E.nigrum var. japonicum, and were identified as 3β-hydroxy-nigrum-21-en (1), 4'-hydroxy-α, β-dihydrochalcone-2'-O-β-D-glucopyranoside (2), 5-(2-phenylethyl)-3-hydroxyphenol-1-O-β-D-glucopyranoside (3), 2', 4'-dihydroxy-chalcone (4), 2'-methoxy-4'-hydroxy-α, β-dihydrochalcone (5), phenylpropionic acid (6), cinnamyl alcohol (7), quercetin (8), (-)-epicatechin (9), quercetin-3-O-α-L-arabinoside (10), hyperoside (11), and 8-methoxyquercetin-3-glucoside (12), respectively. Conclusion Compound 1 is a new triterpenoid and compound 2 is a new dihydrochalcone.

国家自然科学基金资助项目（81260632）