[关键词]
[摘要]
目的 采用HPLC测定山楂中熊果酸、齐墩果酸的量,比较不同产地山楂中熊果酸和齐墩果酸量的差异。方法 利用Waters Symmetry C18色谱柱(250 mm×4.6 mm,5 μm)在流动相为乙腈-甲醇-0.5%乙酸铵(69:12:19),体积流量为1 mL/min,检测波长为210 nm、柱温为30℃的色谱条件下对10个不同产地的山楂进行测定,并对测定结果进行方差分析和聚类分析。结果 熊果酸、齐墩果酸分别在0.078~0.780 mg/mL,r=0.999 8;0.016 9~0.169 mg/mL,r=0.999 6,呈良好的线性关系,加样回收率分别为102.6%、100.2%,均符合《中国药典》2010年版的要求。结论 该测定方法简便快速、重复性好,能够准确测定山楂中熊果酸和齐墩果酸的量;江苏、河北的山楂质量较佳,可以作为欣脉胶囊处方中山楂药材的固定来源。
[Key word]
[Abstract]
Objective To establish an HPLC method for the content determination of ursolic acid and oleanolic acid in hawthorn (Crataegi Fructus) from different regions, whose differences were compared. Methods Waters Symmetry C18 column (4.6 mm×250 mm, 5 μm) was used with the mobile phase of acetonitrile-methanol-0.5% ammonium acetate (69:12:19) at a flow rate of 1 mL/min. The detection wavelength was set at 210 nm and the column temperature was 30℃. To determine the content of hawthorn from 10 different regions, variance analysis and cluster analysis were carried out for the results. Results The linear ranges of ursolic acid and oleanolic acid were 0.078-0.780 mg/mL (r=0.999 8, n=6) and 0.016 9-0.169 mg/mL (r=0.999 6, n=6), respectively. The average recoveries of the two components were 102.6% and 100.2%, in accordance with the determination requirement in Chinese Pharmacopoeia 2010 . Conclusion This method is simple, quick, accurate, and has better reproducibility for the determination of triterpene compounds in hawthorn. And hawthorn from Chengde and Jiangsu provinces is better. It could be used as the origin of hawthorn in the prescription of Xin mai Capsule.
[中图分类号]
[基金项目]
江苏省科技成果转化项目(BA2012020)