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[摘要]
目的 探讨苦参素在诱导肝星状细胞HSC-T6凋亡过程中的作用,以及对端粒酶及其亚单位端粒酶逆转录酶(rTERT)mRNA表达的影响。方法 将不同质量浓度的苦参素与HSC-T6细胞共培养不同时间后,MTT法检测苦参素对HSC-T6细胞的生长抑制作用;流式细胞术检测苦参素对HSC-T6细胞凋亡的影响;TRAP-PAGE-银染法检测苦参素作用后HSC-T6细胞中端粒酶活性的改变;RT-PCR检测苦参素对HSC-T6细胞rTERT mRNA表达的影响。结果 苦参素能显著抑制HSC-T6细胞生长、诱导细胞凋亡、降低端粒酶活性,并抑制rTERT mRNA表达。结论 苦参素对HSC-T6细胞增殖具有抑制作用,可能与抑制细胞端粒酶及其亚单位rTERT的活性有关。
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[Abstract]
Objective To investigate the induction effect of oxymatrine on the apoptosis process in rat hepatic stellate cell line HSC-T6 and to define its impact on telomerase activity and mRNA expression of subunit telomerase reverse transcriptase (rTERT). Methods HSC-T6 cells were cultivated with different concentration of oxymatrine for different time periods. Effect of oxymatrine on the growth inhibition of HSC-T6 cells was analyzed by MTT assay. Apoptosis of HSC-T6 cells was detected by flow cytometry. Telomerase activity was determined by TRAP-PAGE-silver staining and the expression of rTERT mRNA was examined by RT-PCR assay. Results Oxymatrine significantly suppressed the growth of HSC-T6 cells and induced apoptosis, and also reduced the activity of telomerase and inhibited the rTERT-mRNA expression in HSC-T6 cells. Conclusion The function that oxymatrine inhibits the proliferation of HSC-T6 cells may be associated with its action on the cellular telomerase and telomerase rTERT-mRNA activity.
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[基金项目]
湖南省卫生厅科研基金课题(132011-037)