[关键词]
[摘要]
目的 探讨金莲花口服液提取工艺。方法 采用HPLC法测定金莲花口服液提取液中的荭草苷和牡荆苷,以二者提取质量浓度为考察指标,L18(37) 正交试验法逆向连续循环低温提取金莲花,确定最佳提取工艺,并与现行金莲花口服液制备标准中的提取工艺比较。结果 逆向连续循环低温提取最佳提取工艺条件:提取温度90℃,提取时间2 h,药材粒径10目,加水倍数20倍。荭草苷的线性范围18.46~295.37 μg/mL(r=0.999 9),牡荆苷的线性范围5.84~93.44 μg/mL(r=0.999 9),荭草苷平均回收率为96.07%,RSD为0.74%(n=6),牡荆苷平均回收率97.73%,RSD为1.42%(n=6)。结论 逆向连续循环低温提取方法稳定可行,提取率高;测定方法准确、灵敏、简便、稳定性和重复性良好,该提取工艺和测定方法可作为金莲花口服液生产工艺及质量控制的参考。
[Key word]
[Abstract]
Objective To explore the extraction process of Trollii Flos Oral Liquid (TFOL). Methods HPLC was used to determine orientin and vitexin in liquid extract of TFOL, using mass concentration of both as index. The orthogonal design L18(37) and reverse continuous cycle cryogenic extraction were used to extract Trolli i Flos, the optimum extraction was determined, compared with the current standard of TFOL extraction process. Results The optimum extraction conditions of reverse continuous cycle cryogenic extraction were as follows: extraction temperature 90℃, extraction time 2 h, powder type 10 mesh, added of 20 times water. The linear ranges of orientin and vitexin were 18.46-295.37 (r=0.999 9) and 5.84-93.44 μg/mL (r=0.999 9), the average recoveries of orientin and vitexin were 96.07%, RSD=0.74% (n=6), and 97.73%, RSD=1.42% (n=6), respectively. Conclusion The technology of reverse continuous cycle cryogenic extraction is stable and feasible, and the extraction rate is high; The measurement method is accurate, sensitive, simple, and stability, and has good reproducibility. The extraction and determination methods could be used as production process and quality control reference of TFOL.
[中图分类号]
[基金项目]