[关键词]
[摘要]
目的 通过对防风抽薹前后生长素(IAA)、赤霉素(GA)和脱落酸(ABA)的测定,明确了防风抽薹前后IAA、GA和ABA的量变化,为进一步研究防风早期抽薹机制提供理论依据。方法 利用HPLC法测定抽薹前后IAA、GA和ABA量,色谱条件为C18 ODS反相柱,柱温35 ℃,检测波长254 nm,体积流量0.7 mL/min,流动相为甲醇-乙腈- 0.7%乙酸(40:15:45)。结果 防风在抽薹期根、茎、叶中IAA、GA的量变化趋势基本相同,抽薹后IAA、GA的量则急剧下降,且抽薹前防风IAA、GA的量明显高于抽薹后。ABA在防风抽薹期根、茎、叶中的量变化趋势基本相同,但与IAA、GA差异较大。抽薹前取样ABA的量虽略有起伏,抽薹后ABA的量急剧上升,且明显高于抽薹前。结论 防风早期抽薹现象与防风内源激素水平有一定的关系,可以初步推断防风内源激素量的变化导致了防风的早期抽薹。
[Key word]
[Abstract]
Objective Through determining the contents of growth hormone (IAA), gibberellic acid (GA), and abscisic acid (ABA) to make the content changes of IAA, GA, and ABA clear before and after bolting time in the roots of Saponikovia cata, so as to provide the theoretical basis for studying the early bolting mechanism in the roots of S. cata. Methods The contents of IAA, GA, and ABA before and after the bolting time were determined by HPLC. The chromatographic conditions were as follows: C18 ODS RP column, temperature: 35 ℃, detection wavelength: 254 nm, volumn flow: 0.7 mL/min, mobile phase: methanol - acetonitrile - 0.7% acetic acid (40:15:45). Results The content variation trend of IAA and GA in the roots, stems, and leaves of S. cata was almost the same during the bolting time. The contents of IAA and GA in the roots of S. cata revealed the downgrade tendency sharply after bolting; The contents of IAA and GA in the roots of S. cata before bolting were obviously higher than those after bolting. The content variation trends of ABA in the roots, stems, and leaves of S. cata were the same during the bolting time, but far more different from IAA and GA. Although there was the little fluctuation of ABA before bolting, the contents of ABA after bolting were sharply upgraded and were obviously higher than those before bolting. Conclusion The phenomenon of early bolting in the roots of S. cata directly relates to the endo phylohormone level, so we may extrapolate that the levels of endo phylohormone could straightly cause the bolting in the roots of S. cata or not.
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[基金项目]
吉林省科技厅资助项目(2007BA709A17)