目的 研究北柴胡Bupleurum chinense地上茎不同分枝对根中皂苷类成分量及根产量的影响。方法 采用HPLC和紫外分光光度法测定北柴胡地上茎不同分枝植株根中柴胡皂苷a、柴胡皂苷d及柴胡总皂苷的量，并统计分析其生长特征参数。结果 地上茎不分枝与2个分枝的北柴胡根中皂苷类成分量及根产量均最高；随着地上茎分枝数的增多，根中皂苷类成分量及根的产量均呈下降趋势。结论 地上茎不同分枝会对北柴胡根中皂苷类成分量及根产量产生影响。

Objective To evaluate the effect of different stem branches of Bupleurum chinense on saikosaponins content in roots and its root yield. Methods The contents of saikosaponin a, saikosaponin d, and total saikosaponins in the roots of B. chinense were determined by HPLC and UV-vis spectrophotometry. And parameters of growth characteristics were analyzed. Results The contents of saikosaponin a, saikosaponin d, and total saikosaponins in the roots of B. chinense and its root yield were the highest when the B. chinense stem had no branch or two branches. And with the increase of stem branch^'s numbers, the content of saikosaponins in the roots of B. chinense and its root yield decreased. Conclusion Different stem branches have a significant effect on the content of saikosaponins in the roots of B. chinense and its root yield.

辽宁省科学技术计划项目（2012010400-401）；国家级大学生创新创业训练计划项目（201210163028）；辽宁省科技厅本溪特色产业基地科技计划项目（2013226050）