目的 研究硬紫草羟基萘醌提取物对脂多糖（LPS）诱导的小鼠腹腔巨噬细胞RAW264.7细胞炎症因子的抑制作用和对苯磺酸（TNBS）诱导的炎症性肠病（IBD）大鼠结肠组织中肿瘤坏死因子-α（TNF-α）表达的影响。方法 对硬紫草羟基萘醌提取物进行提取分离，采用ELISA法测定硬紫草羟基萘醌提取物对LPS诱导的RAW264.7细胞释放的TNF-α和白细胞介素-1β（IL-1β）的抑制作用；用TNBS法制备IBD大鼠模型，造模24 h后ig给药，连续给药7 d，于末次给药24 h后处死大鼠，采用免疫组织化学法检测大鼠结肠组织中TNF-α的表达。结果 硬紫草羟基萘醌提取物对LPS诱导的RAW264.7细胞释放的TNF-α和IL-1β具有抑制作用；与模型组相比，硬紫草羟基萘醌提取物可降低TNBS诱导的IBD大鼠结肠组织中TNF-α的表达。结论 硬紫草羟基萘醌提取物对TNF-α和IL-1β炎症因子具有不同程度的抑制作用，并可抑制TNBS诱导的肠道炎症反应，这种抑制作用是通过降低TNF-α的表达来实现的。

Objective To study the effect of hydroxynaphthoquinone extract from Lithospermum enythrorhizon on the inflammatory cytokines in lipopolysaccharide (LPS)-induced peritoneal macrophage RAW264.7 cells of mice and on the expression of tumor necrosis factor-α (TNF-α) in the TNBS-induced rat model of inflammatory bowel disease (IBD). Methods The hydroxynaphthoquinone fraction was extracted from L . enythrorhizon. The inhibition of hydroxynaphthoquinone on the inflammatory cytokines was tested by ELISA method. The IBD rat model was induced with TNBS. After 24 h of the model induction, the rats were ig administered for 7 d, After 24 h of the last administration, all rats were killed and the expression of TNF-α in colon tissues was evaluated by immunohistochemistry. Results The hydroxynaphthoquinone extract from L . enythrorhizon had the inhibition on TNF-α and interleukin-1β (IL-1β) in LPS-stimulated RAW264.7 cells. Compared with the model group, the expression level of TNF-αin the colon tissue of IBD rats induced by TNBS was significantly lower in hydroxynaphthoquinone extract groups. Conclusion Hydroxynaphthoquinone extract from L . enythrorhizon has the inhibition on TNF-α and IL-1β, which provides a support for the development of hydroxynaphthoquinone extract from L . enythrorhizon into a therapeutical agent.

国家科技重大专项资助项目（2011ZX09102-006-04）