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[摘要]
目的 利用特异性引物对浙贝母Fritillaria thunbergii进行特异性PCR鉴定。方法 回收RAPD扩增筛选到的浙贝母分子标记ZB1基因,将其连接进入T-载体克隆并测序。根据测序结果设计一对特异性引物P2/P3,以贝母基因组DNA为模板进行特异性PCR扩增。结果 特异性PCR鉴定结果为浙贝母在约750 bp处出现特异性条带,其他贝母品种则未出现条带。 结论 浙贝母特异性PCR鉴定方法操作简单,准确、灵敏、重复性好,具有较好的应用前景。
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[Abstract]
Objective To identify Fritillaria thunbergii using special primer by specific PCR. Methods Gene footprint of F. thunbergii named ZB1 was screened from RAPD amplification. Reclaimed ZB1 gene was inserted into T-vector to be cloned and sequenced. One pair of specific primers P2/P3 were designed according to the ZB1 sequence, and applied in specific PCR reaction using genome DNA of F. thunbergii as template. Results A specific band around 750 bp was detected in F. thunbergii, while nothing appeared in other varieties. Conclusion The method is convenient, reproducible, and precise, with broad application prospects.
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