[关键词]
[摘要]
目的 研究油橄榄Olea europare叶抗糖尿病有效部位的药效物质基础。方法 利用反相中压制备液相色谱技术进行分离纯化,并应用现代波谱技术鉴定化合物的结构。结果 从油橄榄叶抗糖尿病活性部位中分离并鉴定得到的5个化合物均为多酚类化合物,分别为脱咖啡酰基毛蕊糖苷(1)、木犀草素-7-O-β-D-葡萄糖苷(2)、6"-O-β-D-吡喃葡萄糖-橄榄苦苷(3)、裂环马钱子苷(4)、橄榄苦苷(5)。结论 化合物1为首次从该植物中分离得到。5种化合物经HPLC检测,按照归一化法计算其质量分数均大于90%,为以油橄榄叶为原料的新药开发提供技术支撑。
[Key word]
[Abstract]
Objective To study the material basis of the anti-diabetic effective fraction in the leaves of Olea europaea. Methods Preparative medium pressure liquid chromatography (PMPLC) was used for the separation and purification of the compounds. Their structures were elucidated by spectral analyses. Results The structures of the compounds were identified by various spectroscopic methods combined with the data from some published literatures. There were five compounds which were extracted from the anti-diabetic effective fraction in the leaves of O. europaea and identified as decaffeoylverbascoside (1), luteolin-7-O-β-D-glucoside (2), 6"-O-β-D-glucopyranosyl oleuropein (3), oleuroside (4), and oleuropein (5). Additionally, decaffeoylverbascoside is isolated from this plant for the first time and the purities of the five compounds were all above 90% by HPLC detection. Conclusion This research provides technological support for the development of new drug based on the leaves of O. europaea.
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[基金项目]
国家自然科学基金资助项目(20974116);中国科学院“百人计划”资助项目