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[摘要]
目的 检测野菊不同部位(花、叶、嫩茎、老茎)中蒙花苷和绿原酸的量,为野菊各部位质量评价提供理论依据和检验手段。方法 采用HPLC-DAD法测定,色谱柱为Thermo C18(250 mm×4.6 mm,5 μm),乙腈-0.05%磷酸为流动相,梯度洗脱,体积流量1 mL/min,柱温30 ℃,检测波长327 nm。结果 蒙花苷在0.15~3.00 μg(r=0.999 0),绿原酸在0.01~0.20 μg(r=0.999 5)与峰面积呈良好的线性关系,平均回收率分别为98.17%、97.99%;野菊叶中蒙花苷量较高,野菊花中绿原酸量较高,茎中各成分量最低。结论 本法操作简便,结果准确,可用于野菊不同部位的质量控制,定量测定结果显示野菊各部位均存在蒙花苷和绿原酸,但质量分数差异较大。
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[Abstract]
Objective To analyze the contents of linarin and chlorogenic acid in different parts such as flowers, leaves, young stems, and old stems of Chrysanthemum indicum. To provide the theoretical basis and analysis method for the quality evaluation of different parts of C. indicum. Methods Thermo C18 column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of acetonitrile-0.05% phosphoric acid solution in the gradient elution by HPLC-DAD. The flow rate was 1 mL/min, the column temperature was set at 30 ℃, and the detective wavelength was 327 nm. Results The linear response of linarin ranged from 0.15 to 3.00 μg (r = 0.999 0), and that for chlorogenic acid was from 0.01 to 0.20 μg (r = 0.999 5), the average recovery was 98.17% and 97.99%, respectively. The content of linarin from the leaves was higher, the content of chlorogenic acid from the flowers was higher, and the content of each component in the stems was the lowest. Conclusion The method is convenient, and the result is accurate and can be used for the quality control of different parts of C. indicum. The results of content determination indicate that there are linarin and chlorogenic acid in all different parts of C. indicum, but the mass fractions were obviously different.
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