目的 通过毛细管区带电泳（HPCE）内标法，比较戊己丸传统汤剂、配方颗粒汤剂、复方颗粒汤剂中盐酸小檗碱、盐酸巴马汀、盐酸药根碱、芍药苷成分量差异。方法 采用HPCE法，以50 mmol/L硼砂缓冲液-甲醇（2：1）的溶液作为缓冲液，工作电压为25 kV，压力进样5 kPa×15 s，电解质封口：5 kPa×10 s；柱温15 ℃。结果 该方法简便、准确，其中盐酸小檗碱、盐酸巴马汀、盐酸药根碱3种生物碱的量在配方颗粒汤剂中明显高于传统汤剂与复方颗粒汤剂，芍药苷则是在传统汤剂中量最高。结论 本法对戊己丸配方和复方颗粒剂在临床的推广以及质量控制具有指导价值。

Objective To study the content difference of berberine hydrochloride, palmatine hydrochloride, jatrorrhizine hydrochloride, and paeoniflorin in Wujiwan traditional decoction, granule prescription, and compound granule using HPCE internal standard method. Methods Capillary zone electrophoresis (CZE) method was used for a complete separation of the components, which was achieved with 50 mmol/L borax buffer-methanol (2:1), constant voltage of 25 kV, pressure injection 5 kPa × 15 s, electrolyle sealing 5 kPa × 10 s, and column temperature of 15 ℃. Results The capillary electrophoresis method is simple and accurate. The contents of the three alkaloids in the granule prescription were obviously higher than those in traditional decoction and compound granule. Conclusion This method is instructive for clinical application and quality control of Wujiwan prescription and compound granule.

国家自然科学基金资助项目（30860364）；贵阳市科技局项目：2012现代药业计划（筑科合同[2012204]44号）