目的 分析附子-炙甘草配伍前后化学组分变化，探讨附子配伍炙甘草减毒的机制。方法 采用HPLC-Q-TOF/MS分别对附子配伍炙甘草前后汤液进行化学对比研究。分别制备附子单煎液与附子炙甘草合煎液供试品溶液，在同一分析条件下，分别建立其HPLC-MS指纹图谱，并采用Q-TOF/MS检测。结果 从附子单煎液中指认出20种成分及其结构；从附子炙甘草合煎液中指认出32种成分及结构，其中4种成分来源于炙甘草，28种成分来源于附子，但附子配伍炙甘草前后汤液中生物碱成分种类和量差别均较大。结论 说明配伍炙甘草可能导致汤液中附子生物碱成分状态的改变，为附子-炙甘草配伍减毒机制的探讨提供了实验依据。

Objective To analyze the changes of chemical ingredients before and after compatibility of Aconiti Lateralis Radix Praeparata (ALRP) and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (GRRPM), and to explore the possible mechanism of toxicity attenuation of their compatibility. Methods The chemical ingredients in the decoction of ALRP and the decoction of compatibility of ALRP and GRRPM were comparatively researched by HPLC-MS. Single decoction of ALRP and compound decoction of compatibility of ALRP and GRRPM were prepared, and their HPLC-MS fingerprints were respectively established and determined by Q-TOF/MS under the same condition. Results Twenty ingredients and their structures were identified from single decoction of ALRP;32 ingredients and their structures were indentified from the decoction of ALRP and GRRPM, among them 4 from GRRPM, 28 from ALRP. And the alkaloid categories and contents of ALRP were significantly different before and after the compatibility with GRRPM. Conclusion The compatibility with GRRPM could change the alkaloid composition in ALRP, which provides the experimental evidences for the toxicity attenuation mechanism of compatibility of ALRP and GRRPM.

国家自然科学基金面上项目（81073070）