目的 建立同时测定裸花紫珠中5个有效成分的超高效液相色谱（UPLC）方法。方法 采用Agilent Eclipse XDB-C₁₈色谱柱（100 mm×3.0 mm，1.8 μm），流动相为乙腈（A）-0.1%甲酸水溶液（B），梯度洗脱，体积流量为0.7 mL/min。检测波长350 nm，柱温40 ℃。结果 木犀草苷、毛蕊花糖苷、木犀草素-4'-O-β-D-吡喃葡萄糖苷、木犀草素、5，4'-二羟基-3，7，3'-三甲氧基黄酮分别在2.44～122.0 μg/mL（r=0.999 8）、9.06～453.0 μg/mL（r=0.999 9）、4.42～221.0 μg/mL（r=0.999 9）、3.36～168.0 μg/mL（r=0.999 8）、2.52～126.0 μg/mL（r=0.999 8）内线性关系良好；平均回收率在98.5%～100.8%。结论 所建立的UPLC法简便快速、重复性良好、结果准确可靠，可作为裸花紫珠药材质量标准控制的方法。

Objective To develop a UPLC method for the stimultaneous determination of five active ingredients in Callicarpa nudiflora. Methods Analysis was performed on an Agilent Eclipse XDB-C18 column (100 mm ×3.0 mm, 1.8 μm) eluted with acetonitrile (A) and 0.1% methanoic acid (B) in a gradient program. The flow rate was 0.7 mL/min, the detection wavelength was 350 nm, and the column temperature was 40 ℃. Results Luteoloside, acteoside, luteolin-4'-O-β-D-glucopyranoside, luteolin, and 5, 4'-dihydroxy-3, 7, 3'-trimethoxyflavone showed a good linearity in the ranges of 2.44－122.0 μg/mL (r = 0.999 8), 9.06－453.0 μg/mL (r = 0.999 9), 4.42－221.0 μg/mL (r = 0.999 9), 3.36－168.0 μg/mL (r = 0.999 8), and 2.52－126.0 μg/mL (r = 0.999 8). The average recoveries, measured at three concentration levels, varied from 98.5%－100.8%. Conclusion The method is simple, accurate, and can be used for the quality control of C. nudiflora.

国家自然科学基金资助项目（81373955）；中国食品药品检定研究院中青年发展研究基金课题（2013WA9）