目的 旨在建立采用HPLC法同时测定罗布红麻和罗布白麻叶中主要黄酮类成分的方法，同时以新疆产2种罗布麻叶为实验材料，拟揭示其与其他产地罗布麻主要黄酮类成分的差异，为罗布麻质量控制提供依据。方法 采用HPLC法，色谱柱为Agilent TC-C₁₈（150 mm×4.6 mm，5 μm），柱温40 ℃，以乙腈-0.1%磷酸溶液（16：84）为流动相，体积流量为1.0 mL/min，检测波长360 nm，进样量10 μL，测定罗布麻叶乙醇提取物中芦丁、金丝桃苷和异槲皮苷的量。结果 芦丁、金丝桃苷和异槲皮苷的线性范围分别为4.96～49.60、0.96～9.60和10.24～102.40 μg/mL，相关系数R²分别为0.999 2、0.999 0和0.997 0；加样回收率分别为98.2%、92.3%和100.1%，RSD分别为1.5%、3.2%和2.7%。结论 本方法操作简单、结果准确，具有较好的重复性和稳定性，可用于同时测定罗布红麻和罗布白麻叶中3种黄酮类成分，同时，由测定结果可知，新疆产与其他产地的罗布麻叶芦丁和异槲皮苷量差异很大，新疆产罗布红麻芦丁质量分数很高，为0.55%，与异槲皮苷量相当，但金丝桃苷质量分数很低，仅0.07%，而新疆产罗布白麻的芦丁和金丝桃苷质量分数均很低。该研究将为罗布麻叶药材及其提取物质量控制提供科学依据。

Objective To establish an HPLC method for the simultaneous determination of the main flavonoids in the leaves of Apocynum venetum and Poacynum hendersonii, and to reveal the differences of flavonoids in Apocyni Veneti Herba located between Xinjiang and other areas in China. Methods The leaves of the two kinds of Apocyni Veneti Herba were extracted with 60% ethanol by reflux extraction. The separation was carried out on an Agilent TC-C₁₈ column (150 mm × 4.6 mm, 5 μm) eluted with the mobile phases of acetonitrile-0.1% phosphoric acid (16:84). The column temperature was 40 ℃, and the flow rate was 1.0 mL/min, the detection wavelength was 360 nm, and the column temperature was 40 ℃. Results The linear ranges of rutin, hyperin, and isoquercitrin were 4.96－49.60 (r = 0.999 2), 0.96－9.60 (r = 0.999 0) and 10.24－102.40 μg/mL (r = 0.997 0), respectively; and the average recoveries (n = 5) were 98.2% (RSD = 1.5%), 92.3% (RSD = 3.2%), and 100.1% (RSD = 2.7%), respectively. Conclusion This method is simple, rapid, accurate, and reproducible, and the results show that the flavonoids in Apocyni Veneti Herba located in Xinjiang are remarkably different from those in the other areas in China. In the leaves of A. venetum located in Xinjiang, the lutin content is high, about 0.55%, close to the isoquercetin content, but the hypercetin content is very low, only about 0.07%, and in the leaves of P. hendersonii, the contents of both lutin and hypercetin are very small. Such study will offer the credible method for the quality control of A. venetum and P. hendersonii.

新疆维吾尔自治区自然科学基金资助项目（2012211A021）