目的 研究阴离子交换树脂分离纯化绵马贯众总多酚的工艺条件。方法 分别以绵马贯众素ABBA、绵马贯众总多酚的比吸附量、洗脱率、质量分数为指标，优选绵马贯众总多酚的最佳纯化工艺。结果 最佳工艺为绵马贯众药材提取液以6 BV/h的体积流量通过201×7氢氧型阴离子交换树脂柱（柱径与柱高比为1：7），树脂饱和后，水洗至中性，再用3%氯化钠的60%乙醇溶液以6 BV/h的体积流量洗脱，洗脱9倍量树脂柱体积。绵马贯众素ABBA及绵马贯众总多酚洗脱率分别为90.1%和91.1%，绵马贯众素ABBA、绵马贯众总多酚的质量分数分别为29.4%、49.2%。结论 采用201×7氢氧型阴离子交换树脂纯化绵马贯众总多酚效率高，成本低，具有广阔的应用前景。

Objective To optimize the separation and purification of the total poly phenol from Dryopteris crassirhizoma with anion-exchange resin. Methods The rates of elution and absorption, contents of dryocrassin ABBA, and total ploy phenol were used as makers to optimize the purification conditions of total ploy phenol. Results The extracting solution of D. crassirhizoma passed through 201×7 hydrogen-oxygen the anion-exchange resin column (column diameter-column height, 1:7) at the rate of 6 BV/h in reverse direction, then the resin column was flushed with water at the rate of 6BV/h to pH value 6－7 in forward direction. At last, the column was eluted at the rate of 6 BV/h to obtain ploy phenol with 9BV 3% salt water in 60% alcohol. The elution ratio of dryocrassin ABBA is 90.1%, and the total ploy phenol is 91.1%. The content of dryocrassin ABBA is 29.4% and the content of the total ploy phenol is 49.2%. Conclusion The separation and purification of the total ploy phenol from D. crassirhizoma with 201×7 hydrogen-oxygen anion-exchange resin can achieve the satisfactory results which have wide application prospects.

山东省科技攻关计划（2012GGB14018）