[关键词]
[摘要]
目的 建立芪骨胶囊HPLC指纹图谱,为评价其质量提供依据。方法 Platisil-C18色谱柱(250 mm×4.6 mm,5 μm);以甲醇-乙腈-水为流动相梯度洗脱;柱温30 ℃;体积流量1.0 mL/min;检测波长270 nm。结果 建立了15批芪骨胶囊的对照指纹图谱,确定了16个共有峰,其中15个归属到各药材,其相似度均大于0.9。通过对照品对照、UFLC-ESI-MS/MS鉴定了其中8个色谱峰的化学成分。通过聚类分析,15批芪骨胶囊可分为3类。结论 该方法操作简便、准确可靠、重复性较好,为芪骨胶囊的质量控制提供了有效手段。
[Key word]
[Abstract]
Objective To establish the HPLC fingerprint of Qigu Capsules for its quality control. Methods Platisil-C18 column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-methanol-water in gradient elution mode. The detective wavelength was 270 nm, the column temperature was set at 30 ℃, and the flow rate was 1.0 mL/min. Results Fingerprints of 15 batches of Qigu Capsules were established and 16 common peaks were identified, 15 of which belonged to herbs. The similarity was more than 0.9. Eight chemical compounds were identified by comparing the reference substance with UFLC-ESI-MS/MS. Sixteen samples were classified into three groups based on cluster analysis. Conclusion The method can be used for the quality control of Qigu Capsules with good precision, accuracy, and reproducibility.
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[基金项目]
福建省科技厅对外合作重点项目(2012I0004)