[关键词]
[摘要]
目的 研究九节菖蒲Anemone altaica根茎的化学成分。方法 采用Diaion HP-20大孔吸附树脂、Sephadex LH-20,硅胶等柱色谱技术对九节菖蒲水溶性成分进行分离纯化,并根据理化性质和波谱数据结合文献对化合物进行结构鉴定。结果 从九节菖蒲根茎水溶性部位分离并鉴定了14个化合物,其中6个酚类化合物:阿魏酸(1)、单阿魏酰酒石酸(2)、绿原酸(3)、丁香酸葡萄糖苷(4)、阿魏酰-6’-O-α-D-葡萄糖苷(5)、阿魏酰-6’-O-β-D-葡萄糖苷(6);2个木脂素类:(+)-异落叶松脂素-9-O-β-D-吡喃葡萄糖苷(7)、(+)-松脂素-4-O-β-D-吡喃葡萄糖苷(8);1个香豆素类化合物:七叶苷(9);5个含氮化合物:2,3,4,9-四氢-1H-吡啶骈 [3,4-b] 吲哚-3-羧酸(10)、苯丙氨酸(11)、腺嘌呤(12)、胸腺嘧啶核苷(13)、腺苷(14)。结论 化合物2~13均为首次从银莲花属植物分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents in the rhizoma of Anemone altaica. Methods The isolation and purification of the compounds were performed by Diaion HP-20 macroporous resin, Sephadex LH-20, and silica gel column chromatography, and their structures were determined by comparing their physicochemical characters and spectral data with literatures. Results Fourteen compounds were obtained, and their structures were identified, including six phenolic compounds: ferulic acid (1), mono-feruloyl-tartaric acid (2), chlorogenic acid (3), glucosyringic acid (4), feruloyl-6'-O-α-D-glucopyranoside (5), and feruloyl-6'-O-β-D-glucopyranoside (6); two lignans: (+)-isolariciresino-1-9-O-β-D-glucopyranoside (7) and (+)-pinoresinol-4-O-β-D-glucopyranoside (8); one courmarin: esculetin (9); and five nitrogen-containing compounds: 2, 3, 4, 9-tetrahydro-1H-pyridine pyrido [3, 4-b] indole-3-carboxylic acid (10), phenylalanine (11), adenine (12), thymidine (13), and adenosine (14). Conclusion Compounds 2—13 are isolated from the plants of the genus Anemone L. for the first time.
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[基金项目]
教育部科学技术研究重点项目(DF2003078)