[关键词]
[摘要]
目的 对黑刺菝葜Smilaxscobinicaulis根茎的化学成分进行研究。方法 运用硅胶、Sephadex LH-20凝胶和MCI Gel等柱色谱方法进行分离纯化,根据化合物的理化性质和波谱数据鉴定化合物结构。结果 从黑刺菝葜70%乙醇提取物的石油醚和醋酸乙酯萃取部位分离得到13个化合物,分别鉴定为芦荟大黄素(1)、甲基异茜草素-1-甲醚(2)、1, 3, 6, 8-四羟基蒽醌(3)、大黄素(4)、8-羟基-1-甲氧基-3-甲基蒽醌(5)、大黄酚(6)、多花二醌A(7)、多花二醌C(8)、24-乙基胆甾- 4-烯-3, 6-二酮(9)、5α, 8α-过氧化麦角甾-6, 22-二烯-3β-醇(10)、(22E, 24R)-24-甲基-5α-胆甾-7, 22-二烯-3β, 5, 6β-三醇(11)、missourin(12)、齐墩果酸(13)。结论 化合物1~13均为首次从该植物中分离得到,其中化合物1~3和5~13为首次从菝葜属植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents in the petroleum ether and ethyl-acetate soluble extract from the rhizome of Smilax scobinicaulis. Methods The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, MCI gel column chromatography, etc. The structures of the isolated compounds were identified by physicochemical property and spectroscopic methods including 1H-NMR, 13C-NMR, and MS spectra. Results Thirteen compounds were identified as aloe-emodin (1), rubiadin-1-methylether (2), 1, 3, 6, 8-tetrahydroxy-9, 10-anthraquinone (3), emodin (4), 8-hydroxy-1-methoxy-3-methyl-9, 10- anthraquinone (5), chrysophanol (6), floribundiquinone A (7), floribundiquinone C (8), 24-ethylcholest-4-ene-3, 6-dione (9), 3β-hydroxy-5α, 8α-epidioxyergosta-6, 22-diene (10), (22E, 24R)-24-methyl-5α-cholesta-7, 22-diene-3β, 5, 6β-triol (11), 6α-hydroxy- A-neo-germmacer-22(29)-en-30-oic (missourin) (12), and oleanolic acid (13). Conclusion Compounds 1—13 are all isolated from this plant for the first time, Compounds 1—3 and 5—13 are isolated from the plants of genus Smilax L. for the first time.
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[基金项目]
国家自然科学基金资助项目(31070540);深圳市科技创新委项目(JCYJ201206153136998)