目的 建立同时测定常山胡柚花Citrus changshan-huyou Y. B. Chang中柚皮苷、橙皮苷、新橙皮苷的RP-HPLC方法。方法 采用HPLC法，色谱柱为安捷伦ZORBAX SB-C₁₈柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.2%磷酸（15∶85）；检测波长为284 nm；体积流量1.0 mL/min；柱温为40 ℃；峰面积外标法定量。结果 柚皮苷、橙皮苷、新橙皮苷分别在28.45～284.5、18.09～180.93、85.86～858.55 ng内呈良好的线性关系，回归方程分别为Y＝1 725.2 X＋6.383 3，r＝0.999 8；Y＝1 764.6 X＋0.416 7，r＝0.999 8；Y＝1 914.7 X＋15.892，r＝0.999 6。平均回收率分别为98.83%、99.05%、98.83%；RSD分别为2.12%、2.30%、1.85%；并按该建立的方法，测得样品中柚皮苷、橙皮苷、新橙皮苷的量。结论 本方法简便、快速、准确、可靠，可为常山胡柚花的质量控制提供参考。

Objective To establish a method for the simultaneous determination for the contents of naringin, hesperidin, and neohesperidin in the flower of Citrus changshanhuyou by RP-HPLC. Methods The HPLC method was adopted with Agilent Zorbax SB-C₁₈ (250 mm × 4.6 mm, 5 μm) column; The mobile phase was acetonitrile-0.2% aqueous phosphoric acid (15︰85) with the flow rate of 1.0 mL/min. The detection wavelength was 284 nm and column temperature was 40 ℃; The peak areas were quantified by external standard method. Results According to the method, naringin linear was within 28.45—284.5 ng, the regression equation was Y = 1 725.2X + 6.383 3 (r = 0.999 8), and the average recovery rate was 98.83%. Hesperidin linear was within 18.09—180.93 ng, Y＝1 764.6 X＋0.416 7 (r = 0.999 8) with the average recovery rate of 99.05%. Neohesperidin linear was within 85.86—858.55 ng, Y = 1 914.7X + 15.892 (r = 0.999 6), and the average recovery rate was 98.83%. According to the established method, the contents of sample, naringin, hesperidin, and neohesperidin were respectively determined. Conclusion The method is simple, rapid, accurate, and reliable, which provides the reference for the quality control for the flower of C. changshanhuyou, and is suitable for the content determination.

浙江省衢州市科技计划项目（20061082）