目的 从25-OCH₃-原人参二醇（25-OCH₃-PPD）非消旋体混合物中分离得到抗肿瘤有效成分20(R)-25-OCH₃-PPD （AD-1）。方法 采用结晶法拆分25-OCH₃-PPD非消旋体混合物中的AD-1，并对所用溶剂的种类、用量及结晶温度等分离条件进行了优化，探讨溶剂的介电常数和25-OCH₃-PPD非消旋体混合物的S/R比例间的关系。结果 25-OCH₃-PPD非消旋体混合物（R：59.34%；S：38.35%），在最优条件下，丙酮26 mL、4 ℃时，可以获得AD-1的产率与纯度分别为38.76%、97.83%。结论 溶剂的电解质范围在20.7左右，低饱和度的溶剂以及低温有利于AD-1的分离。

Objective To separate antitumor constituent AD-1 [20(R)-25-OCH₃-protopanoxadiol (PPD)] from non-racemic enatiomeric mixture. Methods A crystallization method has been developed to separate AD-1 from 25-OCH₃-PPD non-racemic enatiomeric mixture, the sorts and amount of solvent and crystallization temperature were optimized to obtain the best separative conditions, and the relationship between the S/R percentage of 25-OCH₃-PPD non-racemic enantiomeric mixture and dielectric constant of the solvent was discussed. Results Under the optimized conditions (acetone 26 mL, 4 ℃), the yield and purity of AD-1 crystals are 38.76% and 97.83% from 25-OCH₃-PPD non-racemic enatiomeric mixture (R: 59.34%; S: 38.35%), respectively. Conclusion The ε values of solvents are about 20.7, and an unsaturated solution and low temperature are beneficial to the separation of AD-1 from 25-OCH₃-PPD non-racemic enatiomeric mixture.

“十一五”国家重大新药创制项目（2009ZX09102-114）；中药标准提取物的工业化色谱制备平台建设（2009ZX09301-012-105B）；国家自然科学基金资助项目（81273389）