[关键词]
[摘要]
目的 研究簇花清风藤Sabia fasciculata枝叶的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20、制备液相等多种色谱技术进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果 从簇花清风藤枝叶95%乙醇提取物中分离鉴定了15个化合物,其中7个五环三萜类化合物:3-氧代-12-烯-28-齐墩果酸甲酯(1)、白桦脂醇(2)、3-氧-Δ11, 13(18)-齐墩果二烯(3)、齐墩果酸(4)、imberic acid (5)、拟人参皂苷RP1(6)、竹节参苷IVa(7);3个黄酮类化合物:槲皮素(8)、芦丁(9)、mutabiloside(10);3个生物碱:5-氧阿朴啡碱(11)、N-p-阿魏酰酪胺(12)、N-反式香豆酰酪胺(13);2个甾体类化合物:β-谷甾醇(14)、β-胡萝卜苷(15)。结论 所有化合物均为首次从该植物中分离得到,化合物1、5、6、7、10为首次从该属植物中分离得到。
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[Abstract]
Objective To investigate the chemical constituents in the dried leaves of Sabia fasciculata. Methods Column chromatography such as silica gel, Sephadex LH-20, and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR and 13C-NMR, and physical constants were used to elucidate their structures. Results Fifteen compounds were isolated from 95% ethanol extracts of S. fasciculata, including seven pentacyclic triterpenoids, such as methyl-3-oxo-olean-12-ene-28-oate (1), betulin (2), 3-oxo-olean-Δ11, 13(18)-diene (3), oleanolic acid (4), imberic acid (5), pseudo- ginsenoside RP1 (6), and chikusetsusaponin IVa (7); three flavonoids, such as quercetin (8), rutin (9), and mutabiloside (10); three alkaloids, such as fuseine (11), N-p-feruloyltyramine (12), and N-trans-coumaroyl tyramine (13); two steroids, such as β-sitosterol (14) and β-daucosterol (15). Conclusion All the compounds are isolated from the plant for the first time. Compounds 1, 5—7, and 10 are isolated from the plants of Sabia Colelbr. for the first time.
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[基金项目]
广西科技攻关项目(桂科攻11107010-3-4);广西卫生厅重点科研课题(重2012056)