目的 建立芙朴感冒灵颗粒中芦丁、橙皮苷和牛蒡苷的测定方法。方法 应用HPLC法测定，色谱柱为Agilent Zorbax SB-C₁₈柱（250 mm×4.6 mm，5 μm）；流动相为甲醇-水，梯度洗脱；体积流量为0.8 mL/min，柱温30 ℃；检测波长280 nm。结果 芦丁、橙皮苷和牛蒡苷的线性范围分别为5.204～130.1 μg/mL，r＝0.999 0；1.819～145.5 μg/mL，r＝1.000 0；43.22～778.0 μg/mL，r＝0.999 9；平均加样回收率分别为98.77%、99.52%、100.84%，RSD分别为1.69%、1.01%、1.59%。结论 该方法简便易行、准确、重复性好，可用于芙朴感冒颗粒的质量控制。

Objective To establish a high performance liquid chromatographic method for simultaneous determination of rutin, hesperidin, and arctiin in Fupo Ganmao Granules. Methods HPLC was applied with the chromatographic condition as follows: The chromatographic column was Agilent Zorbax SB-C₁₈ (250 mm × 4.6 mm, 5 μm) at 30 ℃; methanol-water as the mobile phase, gradient elution; flow rate was 0.8 mL/min and the detection wavelength was 280 nm. Results Linear relationships were 5.204－130.1 μg/mL (r = 0.999 0), 1.819－145.5 μg/mL (r = 1.000 0), and 43.22－778.0 μg/mL (r = 0.999 9), respectively. The average recoveries were 98.77% (RSD = 1.69%), 99.52% (RSD = 1.01%), and 100.84% (RSD = 1.59%). Conclusion The results show that the method is simple, accurate, and repeatable, and it is suitable for the simultaneous determination of rutin, hesperidin, and arctiin in Fupo Ganmao Granules.