目的 研究民间药用植物小花八角Illicium micranthum果实中具有抗氧化活性的有效成分。方法 使用DPPH活性测试方法进行抗氧化活性筛选；利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱等多种色谱技术进行化合物的分离、纯化，并根据理化性质和波谱数据鉴定化合物结构。结果 小花八角果实甲醇提取物的醋酸乙酯萃取层抗氧化活性最好，从中共分离得到14个化合物，分别鉴定为gynurenol（1）、β-榄醇（2）、epi-carrisone（3）、6-羟基-桉烷-4(14)-烯（4）、β-桉醇（5）、柳杉醇（6）、1R, 2R, 4R-三羟基薄荷烷（7）、2-isopropenyl-5-methyl-cyclohexanol（8）、(?)-isopulegol（9）、4-isopropenyl- 1-methyl-cyclohexanol（10）、棕榈酸（11）、三十烷酸（12）、对甲氧基苯甲酸（13）、β-胡萝卜苷（14）。结论 除化合物14外，其他化合物均首次从该植物果实中分离得到，其中化合物1～4、7、8为首次从八角科植物中分离得到。

Objective To study the chemical constituents with anti-oxidative activity from the fruits of Illicium micranthum. Methods DPPH Radical scavenging assay was used for screening the active fractions. The chemical constituents were isolated and purified by various column chromatographic methods such as silica gel and Sephadex LH-20. The structures were identified by physicochemical properties and spectral analyses. Results The ethyl acetate layer of methanol extract from the fruits of I. micranthum showed anti-oxidative activity, from which 14 compounds were separated. Their structures were identified as gynurenol (1), β-elemol (2), epi-carrisone (3), 6-hydroxy-eudesm-4(14)-ene (4), β-eudesmol (5), trans-eudesmane-4, 11-diol (6), 1R, 2R, 4R-trihydroxy-p- menthane (7), 2-isopropenyl-5-methyl-cyclohexanol (8), (?)-isopulegol (9), 4-isopropenyl-1-methyl-cyclohexanol (10), palmitic acid (11), triacontanoic acid (12), p-hydroxy-benzoic acid (13), and β-daucosterol (14). Conclusion Except for compound 14, all the other compounds are isolated from the fruits of I. micranthum for the first time, while compounds 1—4, 7, and 8 are isolated from the plants in Illiciaceae family for the first time.

国家自然科学基金资助项目（31200260，31100256）；中央民族大学“985”工程项目（MUC98504-14，MUC 98507-08）；中央民族大学“111引智工程”项目（B08044）；中央民族大学大学生创新创业训练计划资助项目（URTP2012110014，GCCX 2013110010）