目的 研究凹叶厚朴愈伤组织的诱导，并对其产生的有效成分厚朴酚与和厚朴酚总量进行检测，以期获得生产药用成分的新途径，补充药材资源的不足。方法 以不同母株种子幼苗的不同营养器官为外植体，采用不同种类及不同质量浓度的植物调节剂诱导愈伤组织，并在继代培养基中加入前体化合物L-苯丙氨酸及D, L, β-苯丙氨酸对愈伤组织进行诱导培养；HPLC法检测不同培养途径获得的愈伤组织中厚朴酚与和厚朴酚量的总和。结果 筛选出最佳愈伤组织诱导培养基为：B₅＋6-BA 2.0 mg/L＋2, 4-D 1.5 mg/L；不同母株种子幼苗诱导的愈伤组织总酚量存在显著差异，质量分数变化范围为0.004%～0.228%；不同营养器官作为外植体诱导的愈伤组织总酚量存在差异，以幼茎为外植体诱导的愈伤组织总酚量最高可达0.25%；继代培养基中添加前体化合物D, L, β-苯丙氨酸，能有效提高愈伤组织总酚量8～10倍。结论 凹叶厚朴愈伤组织中含有少量的厚朴酚及和厚朴酚，不同的母株种子幼苗及不同幼苗器官诱导的愈伤组织总酚量都有差异，D, L, β-苯丙氨酸能有效促进愈伤组织中总酚的合成。

Objective To study the callus induction and the content of active components magnolol and honokiol from Magnolia officinalis subsp. biloba and to find a new way to obtain medicinal ingredients in order to supplement the medicinal resources. Methods Different vegetative organs from different provenances were adopted as explants, and different kinds of plant regulators with different concentration were used to induce the callus. The precursor compounds, L-phenylalanine and D, L, β-phenylalanine, were added into the subculture medium to induce the synthesis of callus; HPLC method was used to detect the total phenolic content of magnolol and honokiol in callus obtained through different culturing ways. Results The optimal callus induction medium was: B₅ + 6-BA 2.0 mg/L + 2, 4-D 1.5 mg/L; The total phenolic content of callus induced from different provenances had a significant difference in the range of 0.004%—0.228%; There was difference of total phenolic content in different regetative organs as explants, the callus cultured from young stems had the highest magnolol and honokiol content of 0.25%; Adding the precursor compound D, L, β-phenylalanine into the subculture medium could effectively improve the total phenolic content of callus by 8—10 times. Conclusion There is little content of magnolol and honokiol in the callus of M. officinalis subsp. biloba, and the synthesis of total phenolic contents are significantly different in the callus from different provenances and different organs. D, L, β-phenylalanine could effectively improve the synthesis of total phenolic content in callus, and this research has the important significance for sustainable utilization of resources of M. officinalis subsp. biloba.

林业公益性行业科研专项经费“金银花、凹叶厚朴新品种创制及利用技术研究”(201104023)；湖南省教育厅学位与研究生教学教改项目（JG2011B027）；湖南省教育厅重点项目（2012A0146）