目的 探讨植物雌激素葛根素促进人成骨样MG-63细胞分化的分子机制。方法 选择兼有2种雌激素受体（estrogen receptor，ER）亚型表达的人成骨样MG-63细胞为研究模型，采用磷酸苯二钠法、ELISA法及小RNA干扰等技术，探讨葛根素对人成骨细胞分化的作用及作用机制。结果 葛根素可通过提高MG-63细胞中碱性磷酸酶（ALP）活性和I型胶原蛋白分泌促进成骨细胞分化成熟，该作用可被纯ER阻断剂ICI 182, 780完全阻断，应用小RNA干扰技术进一步证实葛根素对成骨细胞ALP活性、I型胶原蛋白分泌的调节作用是由ERα和ERβ共同介导的。结论 葛根素对成骨细胞分化的调节是经ER途径，由ERα和ERβ共同介导。

Objective To investigate the molecular mechanism for promotion of puerarin on the differentiation in human osteoblast-like MG-63 cells. Methods Human osteoblast-like MG-63 cells containing two estrogen receptor (ER) isoforms were selected as the suitable model for this study. Disodium phenyl phosphate method, enzyme-linked immnosorbent assay (ELISA), and small interfering double-stranded RNAs (siRNA) technologies were performed to investigate the effect of puerarin on the differentiation in human osteoblast-like MG-63 cells and its molecular mechanism. Results The promotion of puerarin on osteoblastic differentiation could be performed by increasing alkaline phosphatase (ALP) activity and type I collagen protein secretion in human osteoblastic MG-63 cells. The above effect of puerarin on osteoblast-derived cells could be completely blocked by ER antagonist ICI 182, 780. Using siRNA technology, it was further demonstrated that the effects of puerarin on ALP activity and type I collagen protein secretion were mediated by both ERα and ERβ. Conclusion The promotion of puerarin on osteoblastic differentiation is through the ER pathway and is mediated via both ER isotypes, ERα and ERβ.

国家自然科学基金资助项目（81273520）；天津市自然科学基金资助项目（12JCZDJC26300）；武警后勤学院科学技术研究项目（WHZ201202）；天津市自然科学基金资助项目（13JCYBJC24400）