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[摘要]
目的 研究抗癌药物黄药子Dioscorea bulbifera的茋类化学成分。方法 采用硅胶柱色谱、ODS、Sephadex LH-20柱色谱以及HPLC制备方法进行分离纯化,通过理化性质及波谱数据分析鉴定了化合物的结构。结果 从黄药子95%乙醇提取物的醋酸乙酯萃取部位中分离得到14个茋类化合物,分别鉴定为2, 7-二羟基-3, 4-二甲氧基-9, 10-二氢菲(1)、2, 5-二羟基-4-甲氧基-9, 10-二氢菲(2)、latifolin(3)、2′, 3-二羟基-4, 5-二甲氧基联苄(4)、4-甲氧基菲-2, 3, 7-三醇(5)、3, 7-二羟基-2, 4-二甲氧基菲(6)、2′, 3-二羟基-5-甲氧基联苄(7)、3, 4′, 5-三羟基-3′-甲氧基联苄(8)、7-羟基-2, 3, 4-三甲氧基菲(9)、3, 3′-二羟基-5-甲氧基联苄(10)、2, 7-二羟基-4-甲氧基-9, 10-二氢菲(11)、2, 7-二羟基-3, 4-二甲氧基菲(12)、2, 7-二羟基-2, 4-二甲氧基-9, 10-二氢菲(13)、4, 7-二羟基-2-甲氧基-9, 10-二氢菲(14)。结论 化合物1~3、5~7、9、12、14均为首次从该植物中分离得到。
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[Abstract]
Objective To study the stilbenoids from the rhizomes of Dioscorea bulbifera. Methods The compounds were isolated and purified by chromatography on silica gel, ODS, Sephadex LH-20 columns and HPLC methods. Their structures were determined by means of physicochemical properties and spectral data analyses. Results Fourteen stilbenoids were isolated from the EtOAC partitioned fraction of 95% EtOH extract in D. bulbifera. They were identified as 2, 7-dihyoxy-3, 4-dimethoxy-9, 10- dihydrophenanthrene (1), 2, 5-dihydroxy-4-methoxy-9, 10-dihydroxyphenanthrene (2), latifolin (3), 2′,3-dihydroxy-4, 5- dimethoxy-bibenzyl (4), 4-methoxyphenanthrene-2, 3, 7-triol (5), 3, 7-dihydroxy-2, 4-dimethoxyphenanthrene (6), 2′, 3-dihydroxy- 5-methoxybibenzyl (7), 3, 4′, 5-trihydroxy-3′-methoxybibenzyl (8), 7-hydroxy-2, 3, 4-trimethoxyphenanthrene (9), 3′, 3′-dihydroxy- 5-methoxybibenzyl (10), 2, 7-dihydroxy-4-methoxy-9, 10-dihydroxyphenanthrene (11), 2, 7-dihydroxy-3, 4-dimethoxyphenanthrene (12), 2, 7-dihydoxy-2, 4-dimethoxy-9, 10-dihydrophenanthrene (13), and 4, 7-dihydroxy-2-methoxy-9, 10-dihydrophenanthrene (14). Conclusion Compounds 1—3, 5—7, 9, 12, and 14 are isolated from the rhizomes of D. bulbifera for the first time.
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[基金项目]
基金项目:国家自然科学基金资助项目(81072535,81273390)