目的 研究不同采收期小叶榕叶的HPLC指纹图谱，为鉴定小叶榕药材提供依据，为确定其最佳采收期提供参考。方法 以对羟基苯甲酸仲丁酯作为参照峰将指纹图谱分为高极性和低极性2部分进行表达。Purospher^? STAR C₁₈色谱柱（250 mm×4.6 mm，5 μm），Guard色谱柱：XB-C₁₈（Welch Materials，5 μm），流动相为0.2%磷酸水溶液（A）-乙腈（B），采用梯度洗脱；体积流量1.0 mL/min；柱温35 ℃；检测波长270 nm（高极性部位）和290 nm（低极性部位）。利用HPLC法测定不同采收期的12个样品的色谱图谱。结果 小叶榕叶中所含成分的各色谱峰均得到了有效分离，在测定的12批药材中，共有55个色谱峰，其中图谱显示有44个峰为12批样品所共有，确定为特征指纹峰，对其中10个共有指纹峰进行了标记；初步发现最佳采收期为秋冬季节。结论 该法建立的HPLC指纹图谱具有可靠性，可为进一步研究制定小叶榕叶的质量标准提供依据。

Objective To study the HPLC fingerprint for the leaves of Ficus microcarpa collected in different harvest periods, and to could provide the basis for the identification of F. microcarpa and the optimization of the best harvest periods. Methods The fingerprint chromatogram could be divided into high polarity part and low polarity part by the peak No. 29, which was identified as reference peak of sec-butyl-4-hydroxybenzoate. Analysis was performed on Purospher^? STAR C₁₈ column (250 mm × 4.6 mm, 5 μm) and Guard Column XB-C₁₈ (5 μm, Welch Materials) with the the 0.2% phosphoric acid water solution (A)-acetonitrile (B) as mobile phase. The gradient elution was carried out. The flow rate was 1.0 mL/min. The column temperature was 35 ℃. The monitoring wavelength of the high polarity part and low polarity part was at 270 and 290 nm, respectively. The chromatograms of 12 batches of traditional Chinese medicinal materials in different harvesrt periods were determined by HPLC, which existed a total of about 55 chromatographic peaks. Among them there were 44 chromatographic peaks in common. The peaks were named as characteristic fingerprint peaks. Results The chemical components contained in the leaves of F. microcarpa had been effectively separated. Ten common fingerprint peaks were labeled from the 44 common peaks. The optimal harvest periods were winter and autumn. Conclusion The established method has a good reliability, which could provide the basis for the quality standard for further study on the leaves of F. microcarpa.

教育部留学回国人员科研启动基金（[2011]1139）；国家自然科学基金青年基金资助项目（30801515）