目的 研究刺五加有效成分的提取工艺及比较不同产地刺五加中有效成分的量。方法 采用HPLC法测定刺五加中紫丁香苷、刺五加苷E和异嗪皮啶。确定提取溶媒，采用正交试验法考察加溶媒量、提取时间和提取次数对提取工艺的影响，确定最佳提取工艺。根据最佳提取工艺，比较不同产地刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的量。结果 正交试验所得的最佳提取条件为加10倍量的50%乙醇，提取3次，每次2 h。黑龙江产的刺五加所含紫丁香苷、刺五加苷E和异嗪皮啶量较高。结论 该提取工艺合理、稳定、可行；不同产地刺五加药材中紫丁香苷、刺五加苷E和异嗪皮啶的量存在一定的差异。

Objective To study the extraction technology for the active constituents from Acanthopanax senticosus and to compare their contents from various habitats. Methods The contents of syringin, eleutheroside E, and isofraxidin in A. senticosus were measured using HPLC method. The extracting menstrua were selected, and the influence of the menstruum dosage, extracting times, and extracting time were investigated using orthogonal design method. Based on the optimal technology, the contents of syringin, eleutheroside E, and isofraxidin in A. senticosu from various habitats were compared. Results The optimal conditions for the extraction technology were as follows: extracting for three times, each time for 2 h with 10 times the amount of 50% ethanol. A. senticosus produced in Heilongjiang province had higher contents of syringin, eleutheroside E, and isofraxidin. Conclusion This extraction technology is reasonable, stable, and feasible. The contents of syringin, eleutheroside E, and isofraxidin in A. senticosus from different habitats have some differences. The multi-index contents of syringin, eleutheroside E, and isofraxidin could reflect the quality of A. senticosus more comprehensively.

国家自然科学基金资助项目（81172943）