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[摘要]
目的 研究穿心莲Andrographis paniculata地上部分的化学成分。方法 采用反相硅胶柱色谱、聚酰胺柱色谱、Sephadex LH-20凝胶柱色谱、反相制备高效液相色谱等技术对穿心莲水提取物进行分离纯化,并根据理化性质和波谱学手段进行结构鉴定。结果 从穿心莲地上部分70%乙醇浸膏的水提物中分离得到15个化合物,分别鉴定为5, 4′-二羟基-7-甲氧基-8-O-β-D-葡萄糖黄酮苷(1)、芹菜素(2)、木犀草素(3)、6, 8-二-C-β-D-葡萄糖白杨素(4)、异高黄芩素(5)、5-咖啡酰基奎宁酸(6)、3, 4-二咖啡酰基奎宁酸(7)、3, 4-二咖啡酰基奎宁酸甲酯(8)、3, 4-二咖啡酰基奎宁酸丁酯(9)、4, 5-二咖啡酰基奎宁酸甲酯(10)、咖啡酸(11)、对羟基桂皮酸(12)、阿魏酸(13)、原儿茶酸(14)、富马酸单乙酯(15)。结论 化合物4~6、9、10、14、15为首次从穿心莲属植物中分离得到。
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[Abstract]
Objective To study the chemical constituents from the aereal parts of Andrographis paniculata. Methods The compounds were isolated and purified by various column chromatographies. Their structures were elucidated by means of chemical evidences and spectral analyses (MS, 1H-NMR, and 13C-NMR). Results Fifteen compounds were isolated and identified as 5, 4′-dihydroxy-7-methoxy-8-O-β-D-glucosyl-flavone (1), apigenin (2), luteolin (3), 6, 8-di-C-β-D-glucosylchrysin (4), isoscutellarein (5), 5-caffeoylquinic acid (6), 3, 4-dicaffeoylquinic acid (7), 3, 4-dicaffeoylquinic acid mehtylester (8), 3, 4-dicaffeoylquinic acid butylester (9), 4, 5-dicaffeoylquinic acid methylester (10), caffeic acid (11), p-hydroxy-coumaric acid (12), ferulic acid (13), protocatechuic acid (14), and ethyl fumarate (15). Conclusion Compounds 4—6, 9, 10, 14, and 15 are obtained from this plants for the first time.
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