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[摘要]
目的 筛选野生蛇足石杉引种驯化的优质种质资源,并建立其分子鉴别方法。方法 对来自福建大田、三明市郊、南靖3个不同种质资源野生蛇足石杉引种驯化,HPLC法分析不同种质资源蛇足石杉中石杉碱甲(Hup A)量;采用蛋白质双向电泳技术,分析不同种质资源蛇足石杉差异蛋白。结果 种质资源来自大田的蛇足石杉适应性较强,植株长势好,成活率为65.5%,且茎叶Hup A量也较高,分别为197.80和87.54 μg/g;遮阳成活率为77.8%,远高于未遮阳的成活率(7.1%);黄土地成活率为75%,远高于水田沙壤土的成活率(18.8%)。不同种质资源蛇足石杉共有15个差异蛋白质点,不同种质资源蛇足石杉的差异蛋白质点丰度差异较大。结论 人工引种栽培时,土壤选择以黄土质为佳,并需要给予必要的遮阳;种质资源来自福建大田的蛇足石杉可以作为蛇足石杉优质种质资源进行引种驯化;利用蛇足石杉差异蛋白质点的丰度差异,可以作为鉴别不同蛇足石杉种质资源的依据。
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[Abstract]
Objective To select the high quality germplasm resources from the wild Huperzi serrata during introduction and domestication and to establish the method of molecular identification. Methods After introduction and domestication, three different germplasm resources of H. serrata from Datian, Sanming suburb, and Nanjing were used to determine the contents of huperzine A (Hup A) by HPLC and to analyze the differential protein by two-dimensional gel electrophoresis of protein. Results H. serrata from Datian showed the strong adaptability in the growth potential, survival ratio of 65.5% with higher contents of Hup A, 197.80 and 87.54 μg/g in the stems and leaves of H. serrata, respectively. The survival ratio of H. serrata was 77.8% under shadow, far higher than that (7.1%) under non-shadow, and was 75% in loessland, far higher than that (18.8%) in sand loam paddy field. There were fifteen differential protein spots in H. serrata from the three different germplasm resources. All the protein spots of H. serrata from different germplasm resources had obvious abundance differences. Conclusion The loessland is more suitable for the growth of H. serrata under shadow by artificial cultivation. The H. serrata with the germplasm resource from Datian, Fujian province is used as high quality one during the introduction and domestication. The different germplasm resources of H. serrata could be identified according to the abundance differences in differential protein spots.
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