目的 研究热毒宁注射液体内抗甲型H1N1流感病毒的作用及其机制。方法 采用甲型H1N1流感病毒滴鼻感染BALB/c小鼠制备肺炎模型，观察热毒宁注射液对甲型H1N1流感病毒感染小鼠平均存活时间、存活率、肺指数、病毒载量及细胞因子的影响。结果 与模型组比较，热毒宁注射液显著提高病毒感染小鼠的存活率、延长小鼠平均存活时间（P＜0.05、0.01）；显著降低病毒感染小鼠肺指数、肺组织病毒载量（P＜0.05、0.01）；提高肺组织γ干扰素（IFN-γ）水平，降低白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）水平。结论 热毒宁注射液对甲型H1N1流感病毒感染小鼠具有一定的保护作用，其作用机制可能与提高IFN-γ水平，降低IL-6、TNF-α水平有关。

Objective To investigate the effect of Reduning Injection (RI) on the resistance to influenza A/H1N1 virus and its mechanism. Methods The pneumonic model of BALB/c mice was established by influenza A/H1N1 virus, and the average survival period, survival rate, lung index, viral load, and cytokine level of mice infected by influenza A/H1N1 virus were observed. Results Compared with the model group, RI significantly increased the survival rate and prolonged the average survival period of mice infected by influenza A/H1N1 virus (P < 0.05, 0.01). RI significantly reduced the lung index, viral load, contents of IL-6 and TNF-α, while increased the content of IFN-γ (P < 0.05, 0.01). Conclusion RI has the certain protective effect on mice infected by influenza A/H1N1 virus. Its mechanism may be related to the improvement of the content of IFN-γ and the decrease of the contents of IL-6 and TNF-α.

现代中药创新集群与数字制药技术平台（2013ZX09402203）