魏惠珍1,郭 强1,冯育林1,张武岗1,杨世林1,金浩鑫2,饶 毅1*.檵木中银椴苷的提取及测定[J].中草药,2014,45():
檵木中银椴苷的提取及测定
Extraction and determination of tiliroside from Loropetalum chinense
  
DOI:
中文关键词:  檵木;银椴苷;质量控制;提取分离  单体
英文关键词:Loropetalum chinense (R. Brown) Oliv.  tiliroside  quality control  extraction and separation  monomor
基金项目:国家科技支撑计划课题(2009BADC3B03)
作者单位
魏惠珍1,郭 强1,冯育林1,张武岗1,杨世林1,金浩鑫2,饶 毅1* 1. 江西中医药大学江西 南昌 330006 2. 中药固体制剂制造技术国家工程研究中心江西 南昌 330006 
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中文摘要:
      目的 研究檵木中银椴苷的提取分离及对银椴苷进行质量控制。方法 采用乙醇回流法提取药材,然后经过大孔树脂、减压硅胶柱色谱、洗脱,得到银椴苷单体,采用Cosmosil C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.1%磷酸水溶液(55∶45),检测波长为320 nm,体积流量1.0 mL/min。结果 银椴苷单体能够很好的分离出来,质量分数为98%,银椴苷在0.041~0.513 μg呈良好的线性关系(r=0.999 7);平均回收率为100.05%,RSD为1.50%;结论 运用此方法能将银椴苷较好的提取分离,同时测定方法不仅操作简单、准确,且重复性好,可用于檵木中银椴苷的测定。
英文摘要:
      Objective To research the separation, extraction, and quality control of tiliroside from Loropetalum chinense. Methods The herbs were extracted by using ethanol reflux extraction, and then through macroporous resin, depressure silica gel column chromatography, elution, tiliroside monomer was obtained. Content was determined by the C18 column, mixture of methanol-0.1% phosphoric acid (55:45) as mobile phase at 1.0 mL/min and UV detection at 320 nm were used for the determination. Results Tiliroside can be a good separation of the monomer, the content was 98%; Tiliroside was better in linear relationship between 0.041-0.513 μg (r = 0.999 7). The recovery rate was 100.05%. RSD = 1.50%. Conclusion Using this method tiliroside can be better extracted and separated, Meanwhile the determination method is very stable and accurate and has good repeatability. which can be used for the quality control of tiliroside from L. chinense.
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