徐步青1, 2,李振中2,张 俊2,王 芬1,刘幸佳1,崔永一3*.不同培养条件对铁皮石斛类原球茎生物反应器培养的影响[J].中草药,2014,45():
不同培养条件对铁皮石斛类原球茎生物反应器培养的影响
Effect of different culture conditions on protocorm-like bodies of Dendrobium candidum in bioreactor culture
  
DOI:
中文关键词:  铁皮石斛  类原球茎  培养条件  生物反应器  增殖系数  多糖
英文关键词:Dendrobium candidum Lindl.  protocorm-like bodies  culture condition  bioreactor  proliferationg coefficient  polysaccharides
基金项目:浙江省中青年学科带头人培养资助项目(2272000004);浙江省温州科技局国际合作项目(H20080049);浙江农林大学科技创新项目(100122);浙江省农业科学院国际合作专项经费资助项目
作者单位
徐步青1, 2,李振中2,张 俊2,王 芬1,刘幸佳1,崔永一3* 1. 浙江农林大学园林学院浙江 临安 311300 2. 浙江农林大学林学基础省级实验教学示范中心浙江 临安 311300 3. 浙江农林大学农业与食品科学学院浙江 临安 311300 
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中文摘要:
      目的 研究不同培养条件对铁皮石斛类原球茎在生物反应器中培养的影响。方法 采用接种量、通气量、蔗糖浓度、光照强度等单因素试验设计,烘干法测定生物量,苯酚-硫酸法测定多糖量,数据采用SPSS16.0软件分析。结果 在生物反应器培养条件下,利于铁皮石斛类原球茎增殖和多糖积累的培养基组分为:1/2 MS基本培养基添加1.0 mg/L NAA、5%椰乳、3%蔗糖,pH值为6.0。接种量为40 g/L,采用孔径为15 μm的多孔喷头,通气量用1.0 L/min和0. 5 L/min交替使用以及光照强度为2 000 lx等培养条件有效地提高类原球茎增殖系数和多糖量。结论 不同的培养条件对铁皮石斛类原球茎的生长、多糖的变化有显著影响,适宜的培养条件对铁皮石斛类原球茎的生物量和主要药用成分的生产具有重要的实践意义。
英文摘要:
      Objective To explore the effect of different culture conditions on protocorm-like bodies (PLBs ) of Dendrobium candidum in bioreactor culture. Methods Inoculation quantity, ventilation, sucrose concentration, and light intensity were taken to carriy out the single factor experiment design. Biomass was determined by drying method, polysaccharide content by phenol-sulfuric acid method and SPSS16.0 software was used for data analysis. Results Under the bioreactor culture, the nutrient media components for the proliferation of PLBs of D. candidum and accumulation of polysaccharide were1/2 MS medium supplemented with 1.0 mg/L NAA, 5% coconutmilk, and 3% sucrose, 6.0 pH, was benefit for the proliferation and polysaccharide accumulation of the PLBs. The culture conditions such as inoculation with 40 g/L,, 15 μm pore porous nozzle, aeration volumes by 1.0 and 0.5 L/min used alternately, and photon flux of 2 000 lx could improve the proliferationg coefficient and polysaccharide contents of PLBs. Conclusion Different culture conditions have the significant effect on the growth of PLBs and the accumulation of polysaccharide. Suitable culture conditions have the very important practical significance to the biomass of PLBs and main medicinal ingredient production of PLBs.
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