张智慧1,张倩茹2,付晓萍2,李凌飞2*.基于16S rDNA序列分析研究根腐病三七根内可培养细菌的多样性[J].中草药,2014,45():
基于16S rDNA序列分析研究根腐病三七根内可培养细菌的多样性
Cultivable bacterial diversity in rotting roots of Panax notoginseng with 16S rDNA sequence analysis
  
DOI:
中文关键词:  三七  根腐病  细菌  RFLP分析  DNA测序  16S rDNA
英文关键词:Panax notoginseng (Burk.) F. H. Chen  root rot  bacteria  RFLP analysis  DNA sequencing  16S rDNA
基金项目:国家自然科学基金项目(30900963);国家科技支撑项目(2011BAI13B01-02);云南省中青年学术和技术带头人后备人才项目(2011CI027);中国科学院“西部之光”人才培养计划项目
作者单位
张智慧1,张倩茹2,付晓萍2,李凌飞2* 1. 云南省农业科学院 药用植物研究所云南 昆明 650223 2. 云南农业大学 食品科学技术学院云南 昆明 650201 
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中文摘要:
      目的 分析根腐病三七根内细菌的多样性。方法 用牛肉膏蛋白胨培养基分离根腐病三七根内的细菌,经细菌通用引物27F/1492R扩增16S rDNA后,分别用Rsa I和Hin6 I限制性内切酶对扩增产物进行酶切,结合限制性片段长度多态性(RFLP)分析方法和DNA测序技术,对分离自根腐病三七根内的细菌进行初步鉴定。结果 根腐病三七根内的细菌分属于8个类群,依占总菌数的比例分别是芽孢杆菌属Bacillus 22.47%、色杆菌属Achromobacter 5.62%、寡养单胞菌属Stenotrophomonas 5.62%、类芽孢杆菌属Paenibacillus 4.49%、鞘氨醇杆菌属Sphingobacterium 1.12%、苍白杆菌属Ochrobactrum 1.12%、不动杆菌属Acinetobacter 1.12%及肠杆菌科的一些属58.43%。结论 泛菌属和芽孢杆菌属是根腐病三七根中的两大优势细菌类群。
英文摘要:
      Objective To analyse the bacterial diversity in rotting roots of Panax notoginseng. Methods Bacterial strains were isolated from the diseased roots of P. notoginseng using beef extract-peptone medium. The 16S rDNA was amplificated by primer 27F/1492R, the product was digested by restriction endonuclease Rsa I and Hin6 I, and PCR-RFLP analysis and DNA sequencing technology were used to identify the bacterial strains in rotting roots of P. notoginseng. Results The bacteria could be divided into eight groups including Bacillus (22.47%), Paenibacillus (4.49%), Sphingobacterium (1.12%), Ochrobactrum (1.12%), Stenotrophomonas (5.62%), Achromobacter (5.62%), Acinetobacter (1.12%), and Enterobacteriaceae (58.43). Conclusion The dominant groups in the rotting roots of Panax notoginseng were identified as genera of Pantoea and Bacillus.
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