目的 研究野菊花中萜类和黄酮类化合物对刀豆蛋白A（Con A）诱导的小鼠免疫性肝损伤的保护作用。方法 将小鼠随机分成对照组，模型组，野菊花各提取部位（CIF-A，主要含萜类化合物；CIF-B，主要含萜类和黄酮类化合物；CIF-C，主要含黄酮类化合物）高、中、低剂量组，联双酯滴丸阳性对照组。小鼠首日上下午和次日下午各ig给药1次，以后每天给药1次，连续7 d，末次给药后，除对照组外，其他组小鼠尾iv Con A制备小鼠免疫性肝损伤模型。检测小鼠血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肿瘤坏死因子-α（TNF-α）、γ干扰素（IFN-γ）的水平；测定肝组织中MDA的量，观察肝脏病理组织学变化。结果 与模型组比较，CIF-A、CIF-B均抑制血清中AST、ALT、TNF-α水平的升高；CIF-C抑制AST、ALT、IFN-γ水平的升高；CIF-B可降低肝组织中MDA的量，明显改善肝组织病变，作用优于CIF-A和CIF-C。结论 野菊花中萜类和黄酮类化合物对Con A致小鼠免疫性肝损伤均具有一定的保肝作用，CIF-B作用更强。

Objective To study the protection of the terpenes and flavonoids from Chrysanthemi Indici Flos (CIF) on immunological liver injury (ILI) induced by concanavalin A (Con A) in mice. Methods Mice were divided into control, model, CIF-A (terpenes), CIF-B (terpenes and flavonoids), and CIF-C (flavonoids in high-, mid-, and low-dose) groups, and with Bifendate Pills as positive control group. Mice were ig administered with the three extracts from CIF each once in the morning and afternoon of the first day and the next afternoon, then once daily for consecutive 7 d. The model of ILI was prepared by iv injection of Con A in the tail of mice in each group except the control group. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), inferon-α (TNF-α), and interferon-γ (IFN-γ) in serum of mice were analyzed, the level of MDA in liver was measured, and the histopathological changes of hepatic tissue were observerd. Results Compared with the model group, CIF-A and CIF-B could obviously inhibit the increased levels of AST, ALT, and TNF-α in serum, while CIF-C could mainly reduce the levels of AST, ALT, IFN-γ, and CIF-B could decrease the level of MDA in the liver tissue with the significant amelioration of liver lesions. The effect of CIF-B was stronger than that of CIF-C and CIF-A. Conclusion Terpenes and flavonoids from CIF have a certain protective effect on the liver of mice with ILI induced by Con A, and CIF-B has stronger effect.

国家自然科学基金面上项目（30973872，81273392）；河南省基础与前沿技术研究项目（09230041010）