目的 研究病理和正常生理状态下淫藤骨痹康方总萜体内代谢指纹图谱差异，阐明其活血作用的药效物质基础。方法 采用HPLC法建立藤骨痹康方总萜在正常生理状态与急性血瘀病理状态下的代谢特征指纹图谱，并对共有峰的数与量进行对比分析。结果 在正常生理状态下，5个批次的含药血浆样品中有21个共有峰，其中3个峰来自于空白血浆，4个峰为总萜的原型成分，14个峰为其代谢产物峰或产生的新成分峰；在急性血瘀病理状态下，含药血浆样品中有21个共有峰，其中6个峰来自于空白血浆，3个峰为总萜的原型成分，12个峰为其代谢产物峰或产生的新成分峰；经对照品对照，52 min处的峰为丹参酮II_A。总萜在正常生理状态与病理状态下的色谱峰的数目、峰形及峰的大小存在一定的差异；病理状态下的色谱峰峰面积明显强于正常生理状态下的色谱峰峰面积，其中有7个共有峰的峰面积具有显著性差异（P＜0.05）。结论 淫藤骨痹康方总萜体内代谢指纹图谱的建立方法可行；不同生理状态下的大多数共有峰的峰面积存在显著性差异；其入血原型成分与代谢新成分可能为其活血作用的药效物质。

Objective To elucidate the material basis for the efficacy of promoting blood circulation by means of comparing the differences in in vivo metabolic fingerprints of total terpenes from Yinteng Gubikang Formula (YGF) under pathological and normal physiological status. Methods The metabolic fingerprints of YGF under pathological and normal physiological status (acute blood stasis) were established by HPLC, and the numbers and quantity of the common peaks were compared and analyzed successfully. Results Five batches of drug-containing plasma samples under normal physiological status had 21 chromatographic peaks, among which 3 peaks stemed from blank plasma, 4 peaks existed the prototypes of the total terpenes, and 14 peaks were metabolite. While under pathological status (acute blood stasis), five batches of the drug-containing plasma samples had 21 chromatographic peaks, among which 6 peaks stemed from blank plasma, 3 peaks existed the prototypes of the total terpenes, and 12 peaks were metabolite. The compound with retention time at 52 min was identified as tanshinone II_A by comparing with reference substance. There were some differences in number, shape, and size of the peaks, which represented in vivo compositions of the total terpenes between the pathological and normal physiological status; The intensity of the characteristic peaks produced under the pathological status were stronger than those under the normal physiological status significantly; Variance analysis showed that there were significant differences (P < 0.05) in peak areas of seven peaks. Conclusion The method for establishing the in vivo fingerprint of the total terpenes from YGF is feasible; There are significant differences in peak areas of most common peaks of total terpenes among different physiological status, and prototypes and metabolites may be the effective substances for promoting blood circulation.

湖南省科技厅项目（2009TP4051-1）；长沙市科技计划项目（k1003034-31）；湖南省高校“中药新药创制与资源综合持续利用”科技创新团队资助（湘教通 [2010] 212号）；国家中医药管理局“药用植物学”、“中药药剂学”重点学科资助（国中医药发 [2009] 30号）；湖南省“中药学”重点学科资助（湘教通 [2011] 76号）；中药有毒物质防控技术湖南省工程实验室项目（201304）