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[摘要]
目的 研究黑豆Glycine max种皮的化学成分。方法 采用大孔树脂、硅胶、ODS、Sephadex LH-20、Toyopearl HW-40、MCI柱色谱及半制备HPLC等方法进行分离纯化,并通过理化性质和波谱学技术(ESI-MS、1H-NMR、13C-NMR)鉴定化合物的结构。结果 从黑豆皮提取物中分离得到11个化合物,分别鉴定为矢车菊素-3-O-葡萄糖苷(1)、咖啡酸甲酯(2)、3-甲氧基-4, 5-二羟基苯甲酸甲酯(3)、原儿茶酸甲酯(4)、原儿茶酸乙酯(5)、3-甲氧基-4-羟基-苯甲酸(6)、3-甲氧基没食子酸(7)、二氢槲皮素(8)、原儿茶酸(9)、原儿茶酸酐(10)、表儿茶素(11)。结论 化合物2~10为首次从该属植物中分离得到。
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[Abstract]
Objective To study the phenolic acids from the seed coats of Glycine max. Methods The chemical constituents in the acid water extract from the seed coats of G. max were isolated and purified by chromatographic methods, including macroporous resin, silica gel, ODS, Sephadex LH-20, Toyopearl HW-40, and MCI columns, as well as semi-preparation HPLC. The structures of the isolated compounds were elucidated based on physicochemical properties and spectroscopies, such as ESI-MS, 1H-NMR, and 13C-NMR. Results Eleven compounds were obtained from the acid water extract and identified as cyanidin-3-O-glucoside (1), methyl caffeate (2), 3-methoxy-4, 5-dihydroxy methyl benzoate (3), protocatechuic acid methyl ester (4), protocatechuic acid ethyl ester (5), 3-methoxy-4-hydroxy benzoic acid (6), 3-methoxy gallic acid (7), dihydroquercetin (8), protocatechuic acid (9), protocateuic acid anhydrite (10), and epicatechin (11). Conclusion Compounds 2—10 are isolated from the plants in Glycine Wild. for the first time.
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[基金项目]
天津市科技支撑计划项目(09ZCKFNC01000)