目的 研究吴茱萸药材水溶性化学成分的HPLC指纹图谱，并应用系统聚类分析和主成分分析建立吴茱萸药材的化学模式识别方法，以期为吴茱萸药材质量控制与品质评价提供参考。方法 色谱柱为Ultimate XB-C₁₈色谱柱（150.0 mm×4.6 mm，3 μm）；流动相为乙腈-水（含0.5%醋酸和0.04%磷酸）混合溶液，梯度洗脱；体积流量0.8 mL/min；检测波长为327 nm；进样量10 μL；柱温：25 ℃。结果 34批吴茱萸药材有27个共有峰，相似度在0.945～0.997。聚类分析能对《中国药典》2010年版规定的3种吴茱萸药材进行有效识别，并且从水溶性化学成分上揭示吴茱萸药材的种质远近关系，主成分分析结果与聚类分析结果类似。结论 建立的方法能够成功应用于吴茱萸药材的质量评价与控制及品种识别，可为制定吴茱萸质量评价与控制模式提供新的参考依据。

Objective To establish a method for HPLC fingerprint and chemical pattern recognition of the water soluble component from Evodiae Fructus, so as to provide the reference for the quality evaluation of Evodiae Fructus. Methods The chromatographic separation was performed on a C₁₈ column (150 mm × 4.6 mm, 3 μm). The mobile phase was a mixture of acetonitrile-0.5% acetic acid and 0.04% phosphoric acid aqueous solution in gradient elution. The flow rate was 0.8 mL/min, the detection wavelength was 327 nm, the injection volume was 10 μL, and the column temperature was 25 ℃. Results The HPLC fingerprint of 34 batches of Evodiae Fructus was set up and 27 common peaks were obtained. The similarity was between 0.945 and 0.997. The three species of Evodiae Fructus in Chinese Pharmacopeia 2010 could be completely discriminated by cluster analysis for the first time. The relationship among the different species of Evodiae Fructus were investigated from the water soluble components. The results of the principal component analysis were similar with the cluster analysis. Conclusion The cluster analysis method could be used for the quality control and comprehensive evaluation of Evodiae Fructus.

国家“十二五”科技支撑计划资助项目（2011BAI04B04）；江西中医药大学校级中医药课题（ZX1013）