目的 研究NaCl胁迫对披针叶黄华Thermopsis laceolata愈伤组织生长及生理特性的影响，拟从细胞水平上探讨其适应盐环境的生理机制。方法 在附加0.2%～1.2% NaCl的继代培养基上对愈伤组织进行胁迫培养，测定其中的可溶性糖、可溶性蛋白质、脯氨酸、丙二醛（MDA）量及超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性，并对其进行分析。结果 当NaCl小于或等于0.8%时，随盐离子质量分数的增加，愈伤组织生长量下降，但与对照均无显著差异，而当NaCl大于0.8%时，愈伤组织生长极为缓慢；可溶性糖和脯氨酸量随NaCl质量分数的升高总体呈先上升后下降趋势，但均高于对照；可溶性蛋白质、SOD、CAT和POD酶活性随NaCl质量分数升高呈先上升后下降趋势；丙二醛量在0.8% NaCl及以下时积累缓慢，而NaCl高于0.8%时急剧增加。结论 披针叶黄华愈伤组织具有适应一定质量分数（≤0.8% NaCl）盐渍生境的能力，其在受到盐胁迫时可以通过增加可溶性渗透调节物质如脯氨酸、可溶性糖类以降低渗透势及增强抗氧化酶活性来缓解盐伤害。

Objective To investgate the effects of NaCl stress on the growth and physiological characteristics of Thermopsis laceolata callus, and to reveal the physiological mechanism of T. laceolata under salty environment at the cellular level. Methods The callus was cultured on the subculture medium with NaCl (0.2%—1.2%), and the contents of soluble sugar, soluble proteins, proline, and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were determined and analyzed. Results The callus growth expressed a decreasing trend with NaCl concentration increasing (≤ 0.8%) but had no significant difference to contrast. Nevertheless, the callus grew very slowly with NaCl concentration increasing (> 0.8%); the contents of soluble sugar and free proline increased firstly and then decreased, but were higher than those of the control group; the content of soluble protein and the activities of SOD, CAT, and POD increased firstly and then decreased; MDA accumulated slowly at salinity (≤ 0.8% NaCl), then increased rapidly at salinity (> 0.8% NaCl). Conclusion T. laceolata callus has an adaptability to salty environment (≤ 0.8% NaCl), and the callus could alleviate the salty injury by increasing the contents of the osmotic regulation substances, such as proline and soluble sugar, decreasing the osmotic potential, and increasing the activities of anti-oxidative enzyme.

河西学院科研创新与应用校长基金项目（hxxyxzjj-201003）；广东韩山师范学院2012年科研团队项目（LT201204）