目的 对阳春砂姜黄素生物合成途径的关键酶二酮辅酶A合成酶（diketide CoA synthase，DCS）基因的编码cDNA序列进行克隆，为研究阳春砂姜黄素生物合成与基因调控奠定基础。方法 结合阳春砂的转录组注释，根据编码区序列设计引物，通过PCR方法克隆阳春砂DCS基因的编码区cDNA。结果 PCR扩增了一个长1 170 bp的基因片段，该片段编码由389个氨基酸组成的DCS。同源性比对结果显示基因编码蛋白与姜黄的DCS具有氨基酸一致性达96%，与水仙等植物的查耳酮合酶（chalcone synthase，CHS）氨基酸一致性达66%～69%。进化树分析结果显示，与姜黄Curcumae Longae具有较近的亲缘关系。结论 首次从阳春砂中克隆DCS基因获得其编码区序列，为分析基因表达特性及其在姜黄素生物合成中的功能奠定基础。

Objective To clone the full length cDNA encoding diketide CoA synthase (DCS) gene which plays an important role in curcumin biosynthesis pathway in Amomum villosum, and to provide the basis for the further studies on biosynthesis and gene regulation of curcumin. Methods According to the annotation of root transcriptome of A. villosum, primers were designed and cDNA of DCS gene was cloned from A. villosum by PCR. Results The complete coding sequence of DCS gene was 1 170 bp and it encoded a protein of 389 amino acids. The deduced DCS amino acid sequence exhibited 96% identity to the DCS of Curcumae Longae and 66%—69% identity to the chalcone synthase (CHS) of Narcissus tazetta. Phylogenetic analysis on the amino acid sequence of DCS with those of other plants showed that DCS was closely related to Curcumae Longae. Conclusion The DCS gene is cloned from A. villosum for the first time. This research lays a foundation for studying the gene expression pattern and regulatory functions of DCS in curcumin biosynthesis.

国家自然科学基金资助项目（30700704）