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[摘要]
目的 研究黑老虎Kadsura coccinea的化学成分及其体外抗氧化活性。方法 利用硅胶柱色谱等方法分离纯化,结合理化性质以及光谱数据鉴定化合物结构,DPPH法检测体外清除自由基能力。结果 从黑老虎95%乙醇提取物的醋酸乙酯萃取部位分离得到14个化合物,分别鉴定为micrandilactone C(1)、3, 4-二甲氧基苯基-α-D-吡喃葡萄糖苷(2)、红景天苷(3)、南五味子木脂素N(4)、原儿茶酸(5)、莽草酸乙酯(6)、莽草酸(7)、5-O-β-D-吡喃葡萄糖基龙胆酸(8)、丁香酸(9)、邻苯二甲酸二正丁基酯(10)、苯甲醇-O-β-D-吡喃葡萄糖苷(11)、4-O-β-D-吡喃葡萄糖基香草酸(12)、丁香醛(13)和莽草酸甲酯(14)。化合物5、6和14具有一定的抗氧化作用。结论 化合物2、3、6、8~14为首次从该植物中分离得到;化合物5、6和14在天然抗氧化剂研发中具有一定的开发前景。
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[Abstract]
Objective To study the chemical constituents and their anti-oxidative activities of Kadsura coccinea. Methods The compounds were isolated and purified by column chromatography. The structures were determined by means of physicochemical properties and spectroscopic analyses. The anti-oxidative activity was studied by DPPH methods. Results Fourteen compounds were separated and identified as micrandilactone C (1), 3, 4-dimethoxy phenyl-α-D-glucopyranoside (2), salidroside (3), schisanhenol (4), protocatechuic acid (5), ethyl shikimate (6), shikimic acid (7), 5-O-β-D-glucopyranosylgentisic acid (8), syringic acid (9), 1, 2-benzenedicarboxylic acid-dibuty ester (10), benzyl-O-β-D-glucopyranoside (11), 4-O-β-D-glucopyranosylvanillic acid (12), syringaldehyde (13), and methyl shikimate (14). Compounds 5, 6, and 14 had the anti-oxidative activities. Conclusion Compounds 2, 4, 6, and 8—14 are firstly isolated from K. coccinea. Compounds 5, 6, and 14 have the certain prospects in the development of natural anti-oxidative agents.
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