[关键词]
[摘要]
目的 建立锁阳种子质量评价技术体系并制定锁阳种子质量分级标准。方法 应用体视显微镜、电子天平和离体胚培养法,对锁阳种子形态、千粒质量、胚率和生活力等进行测定,应用数理统计方法分析出划分种子等级的主要指标和参考指标,运用标准差法制定锁阳种子质量分级标准。结果 种子长1.03~1.63 mm,宽为0.69~1.23 mm;种仁长0.61~1.05 mm,宽0.50~0.90 mm;种子形态指标之间极显著相关(P<0.01),形态指标与千粒质量之间显著相关(P<0.05),而与生活力和胚率相关性不显著(P>0.05),千粒质量与生活力和胚率不相关(P>0.05),但生活力和胚率极显著相关(P<0.01);生活力是锁阳种子质量分级的主要指标,净度、千粒质量和水分是种子质量分级的参考指标。结论 1级种子生活力不低于98%,净度不低于93%,千粒质量不低于0.8 g,含水量不高于8%;2级种子生活力95%~98%,净度不低于86%,千粒质量不低于0.7g,含水量不高于8%;3级种子生活力90%~95%,净度不低于80%,千粒质量不低于0.5 g,含水量不高于8%;达不到3级种子标准的为不合格种子。
[Key word]
[Abstract]
Objective To establish the system of the quality evaluation technology for the seeds of Cynomorium songaricum and to draw up the quality grading standard for the seeds of C. songaricum. Methods A stereomicroscope, electronic balance, and the method of in vitro embryo culture were used to measure the morphology, grain, and embryo rate and viability for the seeds of C. songaricum. The method of mathematical statistics were adopted to analyze the main index and reference index of dividing grades of seed; The method of standard deviation was applied in developing the seed quality grading standard. Results Seeds were 1.03—1.63 mm long and 0.69—1.23 mm wide, kernel was about 0.61—1.05 mm long and 0.50—0.50 mm wide. There was a significant correlation among seed morphological indexes (P < 0.01), and there was also a significant correlation between the morphological index and thousand-grain weight (P < 0.05). There was no significant correlation among the morphological index, viability, and embryo rate (P > 0.05) and there was no correlation among the thousand-grain weight, viability, and embryo rate (P > 0.05), while there was a significant correlation between the viability and embryo rate (P < 0.01); the viability was the main index in the quality grading for the seeds of C. songaricum; while the clarity, grain, and water were the reference indicators of quality grading of the seeds. Conclusion The seed quality of each grade should reach the following requirements. For the first grade seeds: seed viability is not less than 98%, purity is not less than 93%, housand-grain weight is not less than 0.8 g, water content is not higher than 8.0%; for the second grade seeds, the seed viability is not less than 95%—98%, the purity is not less than 86%, the thousand-grain weight is not less than 0.7 g, water content is not higher than 8.0%; for the third grade seeds, seed viability is not less than 90%—95%, purity is not less than 80%, thousand-grain weight is not less than 0.5 g, water content is not higher than 8.0%; seeds that do not reach the standard of the third level are unqualified ones.
[中图分类号]
[基金项目]
甘肃省科技支撑项目(2GS035-A43-048-04);甘肃省中小企业创新基金(1110FCCG112);甘肃省高校研究生导师项目(0809-07);甘肃省高校河西走廊特色资源利用省级重点实验室项目(XZ0707);河西学院校长基金项目(XZ201001)