目的 比较莪术醋制前后对复合因素致大鼠肝纤维化的影响及其作用机制。方法 大鼠sc 40%CCl₄橄榄油溶液合并ig乙醇、饲喂高脂饲料制备肝纤维化模型，持续给予诱导剂7周。实验设对照组、模型组、秋水仙碱（0.2 mg/kg）阳性对照组、生莪术（0.95、1.90 g/kg）给药组、醋莪术（0.95、1.90 g/kg）给药组，各给药组于造模同时给予相应药物，每天1次，连续给药8周。检测大鼠血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、总胆红素（TBiL）、透明质酸（HA）、层粘连蛋白（LN）水平，肝脏指数，肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、羟脯氨酸（Hyp）的量。HE染色观察肝组织病理学改变，Masson染色观察肝纤维化程度。结果 与对照组比较，模型组出现典型的肝纤维化病理改变，血清ALT、AST、TBiL、HA、LN水平显著升高，肝组织Hyp、MDA的显著增加，SOD活性显著降低。与模型组相比，生莪术、醋莪术给药组均可不同程度地改善大鼠肝纤维化程度，醋莪术作用效果明显优于生莪术，且其高剂量组有明显的治疗优势。结论 莪术醋制后抗复合因素所致大鼠肝纤维化作用显著增强。

Objective To investigate the effects of Curcuma Rhizoma before and after being processed with vinegar on hepatic fibrosis in rats induced by CCl₄ composited factors and to discuss their mechanism. Methods Rat models of hepatic fibrosis were established by sc injection of 40% CCl₄ olive solution and ig administration with alcohol and high fat food for seven weeks. The rats were randomly divided into control, model, colchicin (0.2 mg/kg, positive), raw Curcuma Rhizoma (RCR, 0.95 and 1.90 g/kg), and Curcuma Rhizoma after being processed with vinegar (CRV, 0.95 and 1.90 g/kg) groups. The rats in each group were administered with the corresponding drugs once daily for eight weeks. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBiL), hyaluronic acid (HA), and laminin (LN) in serum, liver index, the activity of superoxide dismutase (SOD), and the contents of malondialdehyde (MDA) and hydroxyproline (Hyp) in liver tissue were determined. Liver pathology and fibrosis were observed by HE staining and Masson staining. Results Compared with the control group, there were classic liver cirrhosis pathological changes in model groups, and the levels of serum ALT, AST, TBiL, HA, and LN and the contents of Hyp and MDA were significantly increased. The activity of SOD was significantly decreased. In comparison with the model group, both RCR and CRV could improve the liver function parameters in different degrees. But the effect of CRV was superior to that of RCR, and the high-dose group of CRV had obvious advantages. Conclusion CRV shows the better prevention and treatment on hepatic fibrosis induced by CCl₄ composited factor in rats.

国家自然科学基金资助项目（81173548，81001641）；国家药品标准提高暨2015版药典研究课题；中国中医科学院中药研究所开放研究课题（2011ZDXK-02）