[关键词]
[摘要]
目的 研究白花碎米荠Cardamine leucantha的化学成分。方法 采用硅胶、聚酰胺、D-101大孔吸附树脂、凝胶柱色谱等方法对白花碎米荠乙醇提取物进行分离纯化,并根据理化性质和波谱数据对化合物进行结构鉴定。结果 从白花碎米荠乙醇提取物中分离鉴定了14个化合物,其中包括3个简单吲哚类化合物:吲哚-3-甲酸(1)、6-羟基吲哚-3-甲酸(2)、吲哚-3-甲酸-6-O-β-D-葡萄糖苷(3);2个香豆素类化合物:七叶内酯(4)、七叶苷(5);7个甾醇类化合物:3-O-(p-香豆酰基)-β-谷甾醇(6)、β-谷甾醇(7)、5α, 8α-过氧麦角甾-6, 22-二烯-3β-醇(8)、5α, 8α-过氧麦角甾-6, 9(11), 22-三烯-3β-醇(9)、7α-羟基-β-谷甾醇(10)、6′-O-棕榈酰-β-胡萝卜苷(11)、胡萝卜苷(12);2个酚类化合物:对羟基苯甲酸(13)、香草酸(14)。结论 所有化合物均为首次从该属植物中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents in the whole herbs of Cardamine leucantha. Methods The chemical constituents were isolated and purified via silica gel, polyamide, D-101 macroperous resin, and Sephadex LH-20 column chromatography. Their structures were determined on the basis of spectral analysis and physicochemical properties. Results Fourteen compounds were isolated and identified, covering three simple indoles: indole-3-carboxylic acid (1), 6-hydroxyindole-3-carboxylic acid (2), and 6-hydroxyindole-3-carboxylic acid-6-O-β-D-glucopyranoside (3); two coumarins: esculetin (4) and esculin (5); seven steroids: 3-O-(p-coumaroyl)-β-sitosterol (6), β-sitosterol (7), 5α, 8α-epidioxyergosta-6, 22-dien-3β-ol (8), 5α, 8α-epidioxyergosta-6, 9 (11), 22-trien-3β-ol (9), sitost-5-ene-3β, 7α-diol (10), β-sitosteryl-3β-glucopyranoside-6′-O-palmitate (11), and daucosterol (12); and two phenolic compounds: p-hydroxybenzoic acid (13) and vanillic acid (14). Conclusion All the compounds are isolated from the plants in Cardamine L. for the first time.
[中图分类号]
[基金项目]
新世纪优秀人才支持计划资助项目(NCET-09-0589);中国博士后科学基金资助项目(20090450775)