[关键词]
[摘要]
目的 研究滇虎榛Ostryopsis nobilis叶的化学成分及其抗氧化活性。方法 采用多种色谱技术进行分离纯化,根据理化性质和波谱数据鉴定其结构。采用改进的DPPH方法对主要成分进行了抗氧化活性研究。结果 从滇虎榛叶95%乙醇提取物中共分离得到23个化合物,分别鉴定为黑麦草内酯(1)、山楂酸(2)、香草酸(3)、3β-(3, 4-dihydroxycinnamoyl)- erythrodiol(4)、dammarenediol II 3-caffeate(5)、松脂素(6)、槲皮素(7)、胡萝卜苷(8)、山柰酚(9)、3, 5-dihydroxy-1, 7-bis (4H-hydroxyphenyl) heptane(10)、赤杨二醇(11)、木麻黄酮二醇(12)、槲皮素-3-O-α-L-阿拉伯糖苷(13)、异槲皮苷(14)、2, 3-二羟基苯甲酸(15)、异槲皮苷-6″-丁酸酯(16)、异槲皮苷-6″-苯甲酸酯(17)、4″-反-香豆酰基-山柰酚-3-O-α-L-鼠李糖苷(18)、4″-顺-香豆酰基-山柰酚-3-O-α-L-鼠李糖苷(19)、山楂酸-28-O-β-D-葡萄糖苷(20)、没食子酸(21)、桦木四醇(22)和肌醇(23)。结论 化合物1~23均为首次从该属植物中分离得到,抗氧化活性测试显示,滇虎榛叶粗提物的抗氧化活性较强,包括4个二芳基庚烷类化合物在内的6个单体化合物也呈现出一定的抗氧化活性。
[Key word]
[Abstract]
Objective To study the chemical constituents of Ostryopsis nobilis and their anti-oxidant activities. Methods The chemical constituents were isolated and purified by various column chromatographic methods. Their structures were identified by physicochemical properties and spectral analyses. Results Twenty-three compounds were isolated from the 95% ethanol extract and identified as loliolide (1), maslinic acid (2), vanillic acid (3), 3β-(3, 4-dihydroxycinnamoyl)-erythrodiol (4), dammarenediol II 3-caffeate (5), pinoresinol (6), quercetin (7), daucosterol (8), kaempferol (9), 3, 5-dihydroxy-1, 7-bis (4H-hydroxyphenyl) heptane (10), alnusdiol (11), casuarinondiol (12), quercetin-3-O-α-L-arabinoside (13), isoquercetin (14), 2, 3-dihydroxylbenzoic acid (15), isoquercetin-6″-butyl acetate (16), isoquercetin-6″-benzoate (17), 4″-trans-p-coumaroyl-kaempferol-3-O-α-L-rhamnoside (18), 4″-cis-p-coumaroyl-kaempferol-3-O-α-L-rhamnoside (19), maslinic acid-28-O-β-D-glucoside (20), gallic acid (21), betulatetraol (22), and L-chiroinositol (23). Conclusion All the compounds are isolated from the plants in this genus for the first time. Diarylheptanoid compounds and other ten monomer compounds exhibit the good scavenging activities against 2, 2-diphenyl-1-picryhydrazyl (DPPH), and O. nobilis extracts show moderate anti-oxidant effects.
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[基金项目]
国家自然科学基金资助项目(30973634);浙江省自然科学基金资助项目(LY12B02007);温州市科技局科技资助项目(Y20100076)