目的 基于代谢组学研究黄芩对干酵母诱导的热病证候模型的影响，揭示黄芩的苦寒性效关系。方法 实验分对照组、模型组、黄芩高和低剂量（生药12、3 g/kg）组。除对照组外，其他3组sc干酵母悬液（10 mL/kg）；黄芩组分别于造模后4、8 h分别ig给予黄芩水提取液各1次，对照组和模型组ig同体积蒸馏水。以热证的生物标志物及其变化规律为切入点，应用代谢组学、UPLC/ESI-TOF-MS法、主成分分析（PCA）和正交偏最小二乘-判别分析（OPLS-DA）法等技术，研究干酵母诱导的热病证候的代谢特性及黄芩的干预作用，进而探讨中药性味与药效的相关性。结果 确定了30个热证特异性生物标志物，鉴定其中1个离子结构为L-苯丙氨酰-L-羟脯氨酸，并与脯氨酸和苯丙氨酸代谢通路等代谢途径相关。黄芩高、低剂量组各对13个离子具有明显的回调作用。结论 从热病证候代谢物组的整体及生物标志物的变化，揭示了黄芩干预热病证候的作用及其苦寒性效关系。

Objective To study the intervention of Scutellariae Radix on the fever syndrome induced by dry yeast based on metabonomics and reveal its property-effect relationship between bitter and cold. Methods There were control, model, low- and high-dose (3 and 12 crude drug g/kg) Scutellariae Radix groups. The rats in all groups were sc dry yeast suspension (10 mL/kg) except control group. The rats in Scutellariae Radix group were ig given water extract from Scutellariae Radix 4 and 8 h once after modeling, and the rats in model and control groups were given the same volume distilled water. From the aspect of biomarkers of fever syndrome and the variation, the characteristics of dry yeast-induced fever syndrome and intervention of Scutellariae Radix were investigated by metabolomics, ultra-performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (UPLC/ESI-TOF-MS), PCA, and OPLS-DA analyses. Results Thirty specific biomarkers were determined, and the structure of L-phenylalanyl-L-hydroxyproline was identified, and it is correlated with metabolic pathway proline and phenylalanine. The rats in both high- and low-dose Scutellariae Radix groups showed the obvious regulation against 13 ions. Conclusion The property-effect relationship between bitter and cold of Scutellariae Radix is represented by studying the changes of the whole metabolites and biomarkers of fever syndrome.

国家重点基础研究发展计划973计划（2007CB512608，2013CB531804）