[关键词]
[摘要]
目的 研究大孔树脂分离纯化广东紫珠中苯乙醇苷类成分的工艺。方法 以连翘酯苷B和金石蚕苷的量之和为考察指标,采用HPLC法考察10种大孔树脂对苯乙醇苷的吸附分离性能,并考察分离纯化苯乙醇苷类成分的最佳工艺条件。结果 以X-5型大孔树脂为吸附剂,优选的纯化工艺为树脂的最佳吸附量为32.79 mg/g,先以4 BV的水洗脱,除去大部分水溶性杂质,再以7 BV 30%乙醇溶液洗脱,体积流量为2 BV/h,所得提取物中苯乙醇苷的量达52.8%(n=3)。结论 该优选的工艺简便可行,可作为分离纯化广东紫珠中苯乙醇苷类成分的有效方法。
[Key word]
[Abstract]
Objective To study the technology for separation and purification of phenylethanoid glycosides from Callicarpa kwangtungensis by macroporous resin. Methods The absorption and separation properties of 10 kinds of absorption resins were studied and the separation and purification technological process of phenylethanoid glycosides from C. kwangtungensis were optimized by HPLC with the total content of forsythiaside B and poliumoside as an index. Results Using X-5 resin column as adsorbent, the optional conditions were as follows: loading qualities were 32.79 mg/g, the resin was washed by 4 BV distilled water to remove impurity and 7 BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2 BV/h, and the content of phenylethanoid glycosides in the extract was 52.8% (n = 3). Conclusion This technology is simple and feasible, and the process with X-5 resin is an effective method to separate and purify phenylethanoid glycosides from C. kwangtungensis.
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[基金项目]
国家青年自然科学基金项目(81102799)