目的 探讨小黄蘑多糖对小鼠肝癌H₂₂移植性实体瘤的抑制作用及其机制。方法 将H₂₂瘤株接种于小鼠，制备移植性实体瘤模型。将60只小鼠随机分为6组：对照组，模型组，环磷酰胺（200 mg/kg）阳性对照组，小黄蘑多糖低、中、高剂量（50、100、200 mg/kg）组，于小鼠接种H₂₂瘤株第2天开始连续ig给药10 d，每天1次。观察给药后小鼠体质量变化，计算肿瘤抑制率、胸腺及脾脏指数；测定血清肿瘤坏死因子-α（TNF-α）、白介素-2（IL-2）、血管内皮生长因子（VEGF）和白蛋白（Alb）的水平；测定肝组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化物酶（CAT）活性以及还原型谷胱甘肽（GSH）和丙二醛（MDA）的量；测定白细胞数。结果 小黄蘑多糖抑瘤率高于30%；与环磷酰胺组相比，小黄蘑多糖各组显著提高小鼠体质量、胸腺指数以及IL-2和白细胞的水平，降低VEGF的水平，不同程度地提高肝组织中SOD、GSH-Px、CAT活性和GSH的量，降低MDA的量。结论 小黄蘑多糖对H₂₂荷瘤小鼠有一定的抑瘤作用，其机制可能与通过提高机体的抗氧化能力、调节免疫功能、抑制肿瘤组织血管生成有关。

Objective To study the inhibition and the mechanism of Hygrophorus lucorum polysaccharide (HLP) on H₂₂ transplanted tumor in mice. Methods The H₂₂ transplanted models of mice were established. Sixty mice were divided into six groups: control, model, cytoxan (CTX, 200 mg/kg) positive control, low-, mid-, and high-dose (50, 100, and 200 mg/kg) HLP groups. On the day 2 after being inoculated with H₂₂ tumor cells, mice were ig given HLP once daily for consecutive 10 d. And then the body weight change, tumor growth inhibitory rate, and spleen and thymus indexes were calculated; the serum levels of TNF-α, IL-2, VEGF, and albumin (Alb) were determined. The activities of SOD, GSH-Px, CAT, and the contents of GSH and MDA in liver homogenates, as well as the number of WBC were detected. Results The tumor growth inhibitory rate of HLP was over 30%. The treatment with HLP significantly increased the body weight, spleen index, and the number of WBC, elevated the serum levels of IL-2, reduced the VEGF, promoted the hepatic SOD, GSH-Px, CAT activities, and GSH content, and decreased the MDA in liver homogenates. Conclusion HLP has an antitumor effect on H₂₂ transplanted tumor in mice, and the possible mechanisms may be due to its antioxidant activity, regulation of immunofunction, and anti-angiogenetic action.

国家自然科学基金资助项目（30760150）