目的 探讨毛茛总苷对人胃癌MGC803细胞血管生成拟态的影响及其机制。方法 采用细胞黏附实验观察毛茛总苷对MGC803细胞黏附作用的影响；通过细胞划痕实验检测毛茛总苷对MGC803细胞迁移能力的影响；通过Matrigel肿瘤细胞类血管生成模型实验观察毛茛总苷对MGC803细胞体外类血管生成的作用；半定量RT-PCR方法检测毛茛总苷对MGC803细胞血管生成拟态相关基因表达的影响。结果 毛茛总苷有效抑制MGC803细胞黏附、细胞迁移以及MGC803细胞介导的体外类血管生成；明显抑制MGC803细胞血管生成拟态相关基因VE-cadherin、sema 4D和integrin β5的表达。结论 毛茛总苷通过抑制血管生成拟态相关基因VE-cadherin、sema 4D和integrin β5的表达来抑制MGC803细胞黏附、细胞迁移和血管生成拟态。

Objective To investigate the effect of total glycosides from Ranunculus japonicus (TGRJ) on vasculogenic mimicry of human gastric cancer MGC803 cell line in vitro and its mechanisms. Methods The effect of TGRJ on the cell adhesion of MGC803 was observed by cell attachment assay. The effect of TGRJ on the cell migration of MGC803 was studied by cell wound healing assay. The function of TGRJ on the tube-like structures formation of MGC803 in vitro was verified by tube formation assay. The effect of TGRJ on the expression of vasculogenic mimicry related genes was assessed by semi-quantitative RT-PCR assay. Results TGRJ effectively inhibited the cell adhesion, cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry in vitro in a dosage-dependent manner. Furthermore, the semi-quantitative RT-PCR showed that TGRJ markedly inhibited the expression of MGC803 vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5. Conclusion TGRJ could effectively inhibit the cell attachment and cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry by down regulating vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5.

苏州市科技支撑计划项目（SS201004）